水稻缙恢21诱变材料遗传变异的SSR分析

为了获得性状优良的水稻种质资源,采用EMS方法对缙恢21进行诱变,M4代获得不同类型的稳定突变材料,选取36份性状明显不同的突变材料进行SSR分子标记分析。从覆盖水稻12对染色体的200对SSR引物中筛选出15对扩增产物具有稳定多态性的引物,检测到59个等位基因,有效等位基因数为46.2574,有效等位基因百分数为78.40%,每对引物检测出3~5个等位基因,平均为3.9333个;遗传多样性指数在0.9951~1.3849之间,平均为1.2071;每个位点的多态性信息量变化于0.8285~0.9794之间,平均为0.9134。聚类分析得出,36份材料的遗传相似系数变幅为0.515~1.000,并在遗传相似系数0.63处将36份材料分为两大类群。

Genetic variation of induced mutants from rice Jinhui 21 as revealed by SSR markers

In order to obtain good traits of rice germplasm resources, the EMS method was used to mutagenize rice Jin-hui21. Different types of stable mutants were obtained in M4 generation. Thirty-six mutants with obvious different traits were selected for SSR molecular marker analysis. Fifteen pairs of primers with stable polymorphism were screened out from 200 pairs of SSR primers covering 12 chromosomes of rice. Fifty-nine alleles were detected, effective number of alleles was 46.2574, and effective allele percentage was 78.40%. The average number of alleles per SSR locus was 3.9333 with a range of three to five. The genetic diversity index varied from 0.9951 to 1.3849 with an average of 0.9134. The polymor-phism information content for the SSR loci varied from 0.8285 to 0.9794 with an average of 0.9134. Genetic similarities a-mong the 36 mutant materials ranged from 0.515 to 1.000. The 36 mutant materials could be divided into 2 groups by u-sing the genetic similarity coefficient of 0.63.