二甲基甲酰胺对猪精液冷冻保存效果的影响

用二甲基甲酰胺(DMF)完全替代甘油,比较不同平衡时间和不同DMF添加量对猪精液冷冻保护效果的影响。结果表明,DMF能完全替代甘油,获得较好的冷冻保护效果。最佳平衡时间为90 min,解冻后精子活力为(44.57±0.72)%,显著高于对照组和其他组(P0.05)。当DMF添加量为5%时,冻后精子活力、活率、线粒体活性、顶体完整率和质膜完整率分别为(49.91±0.39)%(、46.51±0.26)%、(47.51±0.52)%(、49.84±0.56)%、(46.30±1.61)%,均显著高于2%、3%、6%DMF添加组(P0.05),但与4%DMF添加组相比,冻后精子活力、活率和质膜完整率差异不显著(P0.05)。本试验结果表明,DMF最适添加量为5%。

Effects of Dimethylformamide on Cryopreservation of Boar Semen

Dimethylformamide(DMF) was added at volume fractions of 2%，3%，4%，5% and 6% to the diluents instead of glycerol used to freeze boar semen completely. The effect of equilibration time and DMF volume fraction on the quality of cryopreservation of boar semen was assessed in experiment. The results showed that DMF could improve boar sperm quality significantly in frozen-thawed process .The best equilibration time was 90 min，its sperm motility was （44.57±0.72）% after freezing and thawing which was significantly higher than other groups(P＜0.05)．Sperm viability， motility， mitochondrial activity，acrosome integrity and membrane integrity for 5% dimethylformamide of boar sperm after freezing and thawing were （49.91±0.39）%，（46.51±0.26）%，（47.51±0.52）%，（49.84±0.56）% and （46.30±1.61）%，and were superior to 2%，3%，6%DMF(P＜0.05). Compared with 4% DMF， sperm viability， motility and membrane integrity were similar to 5% DMF after freezing and thawing(P＞0.05)．To sum up，DMF could replace glycerol completely，and the volume fraction of 5% DMF showed a better effect on the quality of boar cryopreservation semen.