Evaluating Inorganic Nitrogen and Rye-Crimson Clover Mixture Fertilization of Spring Broccoli and Lettuce by 15Nitrogen Tracing and Mass Balance

ABSTRACT

Broccoli (Brassica oleraceaL. var. italica) and lettuce (Latuca sativaL.) were grown under greenhouse conditions with nitrogen (N) from a cover crop mixture of rye (Secale cerealeL.) and crimson clover (Trifolium incarnatumL.) and ammonium nitrate (NH₄NO₃). Individual cover crop species were produced with non-enriched or enriched (5 atom % NH₄ ¹⁵NO₃) Hoagland Nutrient Solutions resulting in enriched rye 0.799% atom % ¹⁵N, 24:1 carbon (C):N ratio] and enriched clover (0.686% atom % ¹⁵N, 19:1 C:N ratio). Cover crops were applied as an equal mixture of rye and clover at 1884, 3768, and 5652 kg·ha^{? 1} dry weight to supply 26, 52, and 78 kg·ha^{? 1} N. Enriched materials were only applied at the 3768 kg·ha^{? 1} rate, either as enriched rye plus non-enriched clover or non-enriched rye plus enriched clover. Additional treatments consisted of an unfertilized control and three NH₄NO₃ fertilizer rates; 112, 224, and 336 kg·ha^{? 1} N for broccoli and 70, 140, and 210 kg·ha^{? 1} N for lettuce. Combination treatments were the standard cover crop rate (3768 kg·ha^{? 1}) plus the lowest N fertilizer rate for each vegetable. Cover crops did not increase yield of either broccoli or lettuce, and contributed only 17% of the N in broccoli and 15% of the N in lettuce. The majority of cover crop ¹⁵N remained in the soil: 54.8% and 81.3% of rye and clover N, respectively, after broccoli harvest; and 68.1% and 79.2% of rye and clover N, respectively, after lettuce harvest. Broccoli plant tissue recoveries were 8.0% of the rye and 11.0 % of the clover ¹⁵N; while lettuce plant tissue recoveries were 6.3% (rye) and 4.1% (clover). Broccoli yield could not be assessed due to lack of floret development, but dry matter accumulation was maximized at 224 kg·ha^{? 1}N. Lettuce yield and fertilizer N recovery efficiency (by mass balance) was maximized at 140 g·ha^{? 1} N.