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Effects of Seasonal Nitrate Flush on Nitrogen Metabolism and Soluble Fractions Accumulation in Two Rice Varieties
Authors:André Marques dos Santos  Elvia Mariam Lis Martinez Stark  Manlio Silvestre Fernandes  Sonia Regina de Souza
Affiliation:1. Plant Nutrition Laboratory, Department of Soils , Universidade Federal Rural do Rio de Janeiro , Rio de Janeiro, Brazil;2. Department of Soils , Universidade Federal Rural do Rio de Janeiro , Rio de Janeiro, Brazil;3. Department of Chemistry-Biochemistry , Universidade Federal Rural do Rio de Janeiro , Rio de Janeiro, Brazil
Abstract:
ABSTRACT

Two rice varieties, ‘Piaui’ (a landrace) and ‘IAC-47’ (an improved variety), were grown in nutrient solution containing 20 mg nitrate (NO3 ?)-nitrogen (N) L? 1 up to 32 days after germination (DAG). After this, a group of plants received 200 mg NO3 ?NL? 1, while the other was kept at 20 mg NO3 ?NL? 1 up to 42 DAG. From 42 until 56 DAG, all plants received 5 mg NO3 ?NL? 1. Plants were collected at 42 and 56 DAG, soluble fractions, nitrate reductase (NR) and GS enzymatic activities were determined. The nutritional history of the plants affected significantly the uptake and use of nitrogen (N), and should be taken into consideration in the studies of N-use efficiency. The variety ‘Piaui’ was more efficient than ‘IAC-47’ in N-uptake use, accumulating more NO3 ? in its tissues at the initial phases of its cycle for subsequent utilization.
Keywords:amino-N  nitrogen assimilation enzymes  nitrate uptake  nitrogen use efficiency  Oryza sativa L.  soluble sugars  traditional varieties
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