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Tolerance of Two Hydroponically Grown Salix Genotypes to Excess Zinc
Authors:Andon Vassilev  Araceli Perez-Sanz  Ann Cuypers  Jaco Vangronsveld
Affiliation:1. Agricultural University of Plovdiv, Department of Plant Physiology and Biochemistry , Plovdiv, Bulgaria;2. Departamento de Investigación en Desarrollo Rural , Instituto Madrile?o de Investigación Agraria y Alimentaria , Alcalá de Henares, Spain;3. Environmental Biology, Centre for Environmental Sciences , Hasselt University , Diepenbeek, Belgium
Abstract:
Woody cuttings from two Salix genotypes (genotype I—clone LUC-31, Salix alba and genotype II- clone STOTT, Salix viminalis) were grown hydroponically for 14 days at increasing concentrations of Zn: control, 50, 100 or 150 μ M Zn. Genotype tolerance to excess zinc (Zn) was evaluated using a root elongation test. The changes in growth, Zn, iron (Fe), copper (Cu) and manganese (Mn) concentrations as well as photosynthetic performance were used as additional evaluation criteria. Photosynthetic pigments concentrations in Zn-exposed cuttings of genotype II decreased as compared with genotype I, which corresponded well with the higher leaf Zn accumulation, decreased Fe concentrations as well as lowered photosynthetic rate. Based on the indicators used, genotype I (Salix alba) was classified as more tolerant to excess Zn than genotype II (Salix viminalis).
Keywords:Salix  zinc  tolerance  photosynthesis  mineral nutrients
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