| 用同源模建方法预测蔷薇科斜条卷叶蛾信息素结合蛋白的三维结构 |
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| 引用本文： | 孙浩,赵雷修,周凡蕊,高慧.用同源模建方法预测蔷薇科斜条卷叶蛾信息素结合蛋白的三维结构[J].安徽农业科学,2013(14):6127-6129,6133. |
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| 作者姓名： | 孙浩 赵雷修 周凡蕊 高慧 |
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| 作者单位： | 西南林业大学理学院,云南昆明,650224;云南中医学院,云南昆明,650500 |
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| 基金项目： | 西南林业大学重点科研基金项目 |
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| 摘 要： | 目的]预测蔷薇科斜条卷叶蛾(Choristoneura rosaceana)信息素结合蛋白的三维结构。方法]用同源模建的方法构建蔷薇科斜条卷叶蛾信息素结合蛋白的三维结构,并分别用Procheck、Verify_3D、Errat、ProSa2003等程序对模建结构的可靠性进行评价。结果]模建结构显示蔷薇科斜条卷叶蛾的信息素结合蛋白由6个α-螺旋和连接这些螺旋的回折构成,底物结合口袋成锥形的特征,6个保守的半胱氨酸形成3个对结构起稳定作用的二硫键。结论]预测结构可靠,为蔷薇科斜条卷叶蛾的研究及虫害的防治奠定了理论基础。
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| 关 键 词： | 同源模建 蔷薇科斜条卷叶蛾 信息素结合蛋白 |
Homology Modeling of Pheromone Binding Protein of Choristoneura rosaceana |
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| Institution: | SUN Hao et al ( Facuhy of Science, Southwest Forestry University, Kunming, Yunnan 650224) |
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| Abstract: | Objective ] The research aimed to predict the three dimension structure of pheromone binding protein Choristoneura rosaceana. Method] The three dimension structure of pheromone binding protein of Choristoneura rosaceana was modeled by homology modeling method. The different evaluation of the model by Procheck, Verify_3D, Errat and ProSa2003 respectively indicates that the model is reliable. Result] The pheromone binding protein of Choristoneura rosaceana consists of 6 α-helices and loops between them. 6 conserved cysteines form 3 disulfide bonds which can stabilize the structure. Conclusion] This study can provide a foundation for the research and pest control of Choristoneura rosaceana |
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| Keywords: | Homology modeling Choristoneura rosaceana Pheromone binding protein |
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