| 正交试验优选黄杞叶总黄酮的最佳提取工艺 |
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| 引用本文： | 宋明明,尚志春,付小雪,于大永,史丽颖,唐玲,王永奇.正交试验优选黄杞叶总黄酮的最佳提取工艺[J].安徽农业科学,2013(14):6210-6212. |
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| 作者姓名： | 宋明明 尚志春 付小雪 于大永 史丽颖 唐玲 王永奇 |
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| 作者单位： | 大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622;大连大学药物研究所,辽宁大连,116622 |
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| 摘 要： | 目的]优选黄杞叶总黄酮的正交提取工艺。方法]在单因素试验的基础上,以黄杞叶总黄酮的收率为指标,采用正交试验法优选出黄杞叶总黄酮的提取工艺条件。结果]黄杞叶总黄酮的最佳提取工艺为:料液比1∶25(W/V,g/ml,下同),乙醇浓度70%,温度85℃,回流提取2次,每次2.5 h;在此条件下,总黄酮的收率为21.21%。结论]该工艺操作简单、稳定、可行。
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| 关 键 词： | 黄杞叶 总黄酮 提取工艺 正交试验 |
Extracting Technology Optimization of Total Flavonoids from the Leaves of Engelhardtia roxburghiana Wal.by Orthogonal Test |
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| Institution: | SONG Ming-ming et al (Institute of Materia Medica of Dalian University, Dalian, Liaoning 116622) |
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| Abstract: | Objective] To optimize the extracting technology of total flavonoids from the leaves of Engelhardtia roxburghiarta Wal.. Method] On the basis of single factor test, using the yield of total flavonoids as indicator, the extracting technology of total flavonoids was optimized by or- thogonal test. Result] The optimum extracting technology was as follows ： solid-liquid ratio 1 ： 25 （ W/V, g/ml, the same as follows）, 70% etha- nol, extracting 2 times at 85 ℃ for 2.5 hours. The yield of total flavonoids was 21.21% under the above condition. Conclusion] The extracting technology is simple, stable and feasible. |
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| Keywords: | Engelhardtia roxburghiana Wal Total flavonoids Extraction technology Orthogonal test |
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