| 脱脂奶溶蛋白琼脂扩散抑制试验检测兔血清中嗜水气单胞菌胞外蛋白酶的抗体 |
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| 引用本文： | 朱杰青,吉传义,陆承平.脱脂奶溶蛋白琼脂扩散抑制试验检测兔血清中嗜水气单胞菌胞外蛋白酶的抗体[J].南京农业大学学报,2001,24(1):118-119. |
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| 作者姓名： | 朱杰青 吉传义 陆承平 |
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| 作者单位： | 南京农业大学动物医学院, |
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| 基金项目： | 国家九五攻关子课题! (NO96 0 0 5 0 3 0 2 ) |
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| 摘 要： | 利用蛋白酶的蛋白特性，结合琼脂免疫扩散试验，建立了脱脂奶溶蛋白琼脂扩散抑制试验的新方法，用以检测免血清中的嗜水气单胞菌（Aeromonas hydrophilla)胞外蛋白酶抗体，同时与琼脂扩散免疫沉淀试验及ELISA试验进行了比较，结果显示，该方法的敏感性优于琼脂扩散免疫沉淀试验，而与ELISA试验相当。
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| 关 键 词： | 嗜水气单胞菌 胞外蛋白酶 抗体 检测 脱脂奶溶蛋白琼脂扩散抑制试验 |
| 文章编号： | 1000-2030(2001)01-0118-02 |
| 修稿时间： | 2000年5月31日 |
Detection of the antibody to extracellular proteases of A hydrophilla by skim milk proteolysis agar diffusion inhabit test |
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| Zhu Jieqing,Ji Chuanyi,Lu Chengping.Detection of the antibody to extracellular proteases of A hydrophilla by skim milk proteolysis agar diffusion inhabit test[J].Journal of Nanjing Agricultural University,2001,24(1):118-119. |
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| Authors: | Zhu Jieqing Ji Chuanyi Lu Chengping |
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| Abstract: | A new method—skim milk proteolysis agar diffusion inhabit test was established according to the proteolysis character of proteases and the agar immunodiffusion test(AIDT).The new method was used to detect the antibody to extrocellular proteases of Aeromonas hydrophila from rabbit serum.And compared with the agar immunodiffusion test and the ELISA,it sh owed that the new method was more sensitive than the AIDT and equal as ELISA. |
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| Keywords: | Aeromonas hydrophilla extrcellular proteases antibody detection |
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