Molecular characterization and expression of the Prostaglandin reductase 1 gene and protein during ovarian development of the giant tiger shrimp Penaeus monodon |
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Authors: | Sirikan Prasertlux Kanchana Sittikankaew Parichat Chumtong Bavornlak Khamnamtong Sirawut Klinbunga |
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Institution: | 1. Collaborative Innovation Center of Deep Sea Biology and College of Life Sciences, Zhejiang University, Hangzhou 310058, PR China;2. Key Laboratory of Marine Ecosystem and Biogeochemistry, The Second Institute of Oceanography, State Oceanic Administration, Hangzhou 310012, PR China;1. Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, PR China;2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, PR China;3. University of Chinese Academy of Sciences, Beijing 10049, PR China;1. Scientific Observing and Experimental Station of Fishery Resources and Environment in the Changjiang River, Freshwater Fisheries Research Center, Wuxi, Shanshui Road 9, 214081, China;2. School of Life Science, East China Normal University, 3663 North Zhongshan Road, Shanghai 200062, China |
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Abstract: | Prostaglandin reductase 1 (PTGR1; also called NADP+-dependent leukotriene B4 12-hydroxydehydrogenase, LTB4DH) is the key enzyme responsible for biological inactivation of prostaglandins and related eicosanoids. In this study, the full-length cDNA of PTGR1 in the giant tiger shrimp (Penaeus monodon) was characterized. PmPTGR1 was 2405 bp in length with an ORF of 1035 bp encoding a polypeptide of 344 amino acids. Interestingly, its 3′ UTR contained the nucleotide sequence (825 bp) that significantly matched positions 3–277 (with 4 amino acid variants) of the deduced P. monodon peritrophin2 protein. PmPTGR1 was more preferentially expressed in ovaries than testes of P. monodon broodstock. In intact broodstock, PmPTGR1 was up-regulated in early cortical rod (stage III) ovaries (P < 0.05) and comparably expressed afterwards (P > 0.05). In eyestalk-ablated broodstock, PmPTGR1 was temporally lower in early cortical rod compared to previtellogenic (I) and vitellogenic (II) ovaries (P < 0.05) and returned to the previous level in mature (IV) ovaries (P < 0.05). More importantly, the relative expression level of PmPTGR1 in each ovarian stage in eyestalk-ablated females was lower than that in intact P. monodon broodstock (P < 0.05). This strongly suggested that eyestalk ablation potentially affects the expression of PmPTGR1 allowing the stimulating effects of prostaglandins and related eicosanoids on vitellogenesis and ovarian maturation of P. monodon. The level of ovarian PmPTGR1 protein seemed to increase during ovarian development in intact broodstock but slightly reduced in mature ovaries in eyestalk-ablated broodstock. Results suggested the possible contribution of PmPTGR1 in ovarian development of P. monodon. |
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