| 猪KISS-1基因克隆、序列分析 |
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| 引用本文： | 臧猛,晁利刚,张志强,台玉磊,王静,武宇晓,杨国宇.猪KISS-1基因克隆、序列分析[J].江西农业学报,2008,20(11):5-7. |
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| 作者姓名： | 臧猛 晁利刚 张志强 台玉磊 王静 武宇晓 杨国宇 |
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| 作者单位： | 河南农业大学,动物生理生化实验室,河南,郑州,450002 |
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| 基金项目： | 河南省重点科技攻关项目
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| 摘 要： | 在猪的不同组织中克隆KISS-1基因，提取总RNA，利用设计的引物进行RT-PCR，PCR产物与pMD-19T载体连接后转化E．coliDH5α，检测阳性克隆并测序；结果：克隆的猪KISS-1基因与牛、人、大鼠、小鼠同源性分别为81．0％、76．8％、71．4％、72．4％；克隆了猪KISS-1基因全长并注册GenBank（Accession．EU934235），猪KISS-1基因在多种组织中表达。
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| 关 键 词： | 猪 KISS-1基因 GPR54 克隆 序列分析 组织分布 |
Molecular Cloning and Sequence Analysis of Porcine Gene KISS-1 |
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| ZANG Meng,CHAO Li-gang,ZHANG Zhi-qiang,TAI Yu-lei,WANG Jing,WU Yu-xiao,YANG Guo-yu.Molecular Cloning and Sequence Analysis of Porcine Gene KISS-1[J].Acta Agriculturae Jiangxi,2008,20(11):5-7. |
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| Authors: | ZANG Meng CHAO Li-gang ZHANG Zhi-qiang TAI Yu-lei WANG Jing WU Yu-xiao YANG Guo-yu |
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| Institution: | ZANG Meng, CHAO Li-gang, ZHANG Zhi - qiang, TAI Yu-lei, WANG Jing, WU Yu - xiao, YANG Guo - yu(Lab of Animal Physiology ＆ Biochemistry, Henan Agricultural University, Zhengzhou 450002, China) |
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| Abstract: | In order to clone and analyze the gene KISS-1 in various tissues of pig,the total RNA was extracted and mRNA sequence of KISS-1 gene was amplified by RT-PCR method which used two designed primers.The PCR products were ligated into the pMD-19Tvector,and then transformed into competent cells of E.coli DH5α.The sequence was analyzed to identify the recombinant plasmid.The identification analysis showed that the KISS-1 nucleotide sequence shared 81.0%,76.8%,71.4% and 72.4% homology with that of Bos taurus,human,rat and mouse respectively,revealing porcine KISS-1 gene had been successfully cloned in the present study.The sequence has been submitted to GenBank(Accession EU934235).The expression of porcine KISS-1 gene in various tissues was found. |
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| Keywords: | GPR54 |
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