一个马铃薯Y病毒山东分离物的分离与鉴定

 从具有典型花叶症状的马铃薯叶片中分离到马铃薯Y病毒(Potato virus Y,PVY)(本文称PVY-SD-TA分离物),扩繁后,提纯病毒,电镜下可观察到700~900 nm×11 nm的病毒粒体,病组织超薄切片观察可见风轮状的内含体结构,寄主反应特性研究表明其能侵染2科13种植物。SDS-PAGE电泳检测病毒编码的外壳蛋白亚基的分子量为33 kDa。以PVY-SD-TA基因组RNA为模板,应用RT-PCR方法和特异引物合成了外壳蛋白基因。对cDNA全序列分析表明,PVY-SD-TA CP基因核苷酸序列与N株系的同源性为96%,与GenBank中登录序列号为AJ390306的O株系分离物的同源性最高,为99%;与国内不同学者报道的PVY中国流行株的同源性分别为96%,97%和98%。通过以上生物学特性和分子水平的研究将PVY-SD-TA鉴定为普通株系(PVY^O株系)。

Isolation and identification of a Potato virus Y isolate in Shandong

An isolate of Potato virus Y (PVY-SD-TA)was isolated from potato leaves with typical mosaic symptoms in Taian, Shandong Province, and maintained in Nicotiana tabacum cv. Samsun. Electron microscope study revealed that the size of the purified virus particle was 700-900 nm×11 nm. Pine-wheel inclusions were found in the infected leaf. The study of host responses to virus infection suggested that the PVY-SD-TA could infect plants of 13 species from 2 families. The molecular weight of the coat protein (CP)subunit was 33 kDa as evaluated by SDS-PAGE. The CP gene was amplified by RT-PCR using the genomic RNA as template and a pair of primers specific to PVY. Sequencing results showed that the homology between PVY-SD-TA CP gene and PVY^N and PVY^O were 96% and 99%, respectively. According to these results, PVY-SD-TA was identified as an isolate of PVY^O strain.