不同来源牛体外胚胎与牛子宫内膜细胞共培养体系的建立

为了初步建立有效的牛胚胎与子宫内膜细胞的体外共培养体系。本实验分别将体外受精第7天后的胚胎和孤雌激活，与培养7天的胚胎与子宫内膜细胞共培养，在48 h和72 h后分别统计体外受精实验组和孤雌激活实验组的孵化率。实验结果表明：在本实验室培养条件下，在与子宫内膜细胞共培养48 h后，体外受精胚胎和孤雌发育胚胎在孵化率上存在显著差异(40±5)%、(25±5)%，(P＜0.05)；与子宫内膜共培养72 h后，孵化率无显著差异(70±8.66)%，(65±5)%，(P＞0.05)。本实验证明了在体外环境下，牛体外胚胎与子宫内膜细胞共培养后能够正常孵化，可以初步建立起牛体外胚胎与子宫内膜细胞的体外共培养体系，并为进一步研究该体系对牛体外胚胎培养质量的影响奠定一些基础。

Establishing an Effective Way to Co-culture the in-vitro Bovine Blastocysts from Different Methods With Their Uterine Endometrium Cells

In this study,it was initiated to establish an effective way to co-culture the bovine blastocysts and their uterine endometrium cells in vitro.Here,total 7 days in vitro fertilized（IVF） or 7 days parthenogenetic（PA） bovine blastocysts were put into the quadripuntal broad with 42,72 h uterine endometrium cells.The results showed that,after being co-cultured with the uterine endometrium cells for 48 h,the hatching rate of IVF blastocysts was significantly higher than that of PA group（40±5）% vs（25±5）%,P 0.05）,but after being co-cultured for 72 h,there was no significant differences of hatching rate between them（70 ± 8.66）% vs（65±5）%,P0.05）.In conclusion,these results suggested that,such kind of in vitro co-culture system between those in vitro produced bovine blastocysts and the uterine endometrium cells,be a potential and effective way for further investigating the quality of bovine embryos.