杂种奶牛POU1Fl、GHR、GHRH-R和CSN1S2基因部分序列PCR-SSCP多态性分析

利用PCR-SSCP技术，对荷斯坦公牛冻精改良的93头杂种奶牛的脑垂体转录因子-1（POU1Fl）基因第6外显子、生长激素受体（GHR）基因第10外显子、生长激素释放激素受体（GHRH-R）基因第2外显子和酪蛋白(CSN1S2) 基因第18外显子进行了多态性研究。结果表明，在检测的4个基因位点中，只有POU1Fl基因第6外显子存在2种基因型，AB、BB基因型频率及A、B基因频率分别为0.0323、0.9677和0.0162、0.9838，未发现AA型个体。该群体在此位点上处于Hardy-Weinberg平衡状态，基因杂合度和多态信息含量分别为0.0318和0.0313。测序显示，与普通黄牛POU1Fl基因序列Y15995相比，A等位基因在POU1F1基因第6外显子的第209个碱基处发生了G→C的碱基颠换，导致Thr/Arg替代，其多态的成因及效应需进一步研究。

Study on PCR-SSCP Polymorphism of POU1Fl,GHR,GHRH-R and CSN1S2 Gene Part Sequence in Hybrid Dairy Cattle

The PCR-SSCP polymorphism of POU1F1 gene exon 6,GHR gene exon10,GHRH-R gene exon 2 and CSN1S2 gene exon 18 were studied in 93 hybrid dairy cattles improved by crossbreeding with Holstein frozen sperm.The results showed that in four examined genes locus,only the POU1Fl gene exon 6 have two genotypes,the genotypic frequencies of AB and BB were 0.0323 and 0.9677 respectively;the allelic frequencies of A and B were 0.0162 and 0.9838,and the individual with genotype AA has not discovered.The population was at Ha...