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| 甜瓜品种GT-1植株再生体系的建立(英文) | |
| 引用本文： | 黄永红,陆璐,陶兴林,赵长增. 甜瓜品种GT-1植株再生体系的建立(英文)[J]. 果树学报, 2006, 23(5): 740-744,F0003 |
| 作者姓名： | 黄永红 陆璐 陶兴林 赵长增 |
| 作者单位： | 1. 广东省农业科学院果树研究所,广州,510640 2. 甘肃农业大学农学院园艺系,兰州,730070;西北师范大学生命科学院,兰州,730070 3. 甘肃省农业科学院蔬菜研究所,兰州,730070 4. 甘肃农业大学农学院园艺系,兰州,730070 |
| 基金项目： | 国家自然科学基金 , 甘肃省生物技术专项基金 |
| 摘 要： | 为了建立高效稳定的甜瓜作物的植株再生体系,研究了甜瓜品种CT-1在16种培养基上的植株再生情况。结果显示:将从5 d龄甜瓜无菌苗上切取的子叶外植体接种在MS+6-BA1 mg／L的不定芽诱导培养基上,黑暗条件培养3 d,再转至光下继续培养,诱导出高效不定芽丛。再经过在MS+6-BA0．05mg/L伸长培养基上培养后,将无根不定苗转到不含激素的MS培养基上进行生根诱导,获得完整植株。通过这种培养程序,获得了高达100％的不定芽诱导率,平均每个外植体上诱导出14株健壮植株。这项研究为甜瓜的遗传转化打下良好的基础。 |
| 关 键 词： | 甜瓜 植株再生 不定芽 |
| 文章编号： | 1009-9980（2006）05-740-05 |
| 收稿时间： | 2006-04-12 |
| 修稿时间： | 2006-04-122006-07-24 |
Establishment of plant regeneration system for Cucumis melon cv. GT-1 |
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| HUANG Yong-hong,LU Lu,TAO Xing-lin,ZHAO Chang-zeng. Establishment of plant regeneration system for Cucumis melon cv. GT-1[J]. Journal of Fruit Science, 2006, 23(5): 740-744,F0003 | |
| Authors: | HUANG Yong-hong LU Lu TAO Xing-lin ZHAO Chang-zeng |
| Affiliation: | 1 Fruit Research Institute, Gnangdong Academy of A gricultural Science, Guangzhou , Guangdong 510640 China; 2 Department of Horticul- ture ,College of Agronomy,Gansu Agricultural University,Lanzhou 730070 China; 3. College of Life Sciences ,Northwest Normal University, Lanzhou ,Gansu 730070 China; 4 Vegetable Research Institute ,Gansu Academy of Agricultural Science ,Lanzhou ,Gansu 730070 China |
| Abstract: | In order to establish a stable and efficient plant regeneration system for Cucumis me/on,plant regeneration of C. me/on cv. GT-1 was studied on 16 culture mediums. The cotyledon explants excised from 5-day-old germfree seedlings were cultured on the MS medium containing 1.0 mg/L 6-BA in darkness for 3 days ,and then further cultured under light condition for induction of adventitious buds. Bud clusters thus generated were transferred onto the MS medium supplemented with 0.05 mg/L 6-BA. The rootless elongated shoots were then transferred to hormone-free MS medium, on which roots were induced and complete plants were obtained. Through the protocol,an adventitious bud induction rate of 100% was obtained and an average of 14 healthy complete plantlets were generated from each explant. The study laid a good foundation for gene transformation of C. me/on. |
| Keywords: | Cucumis melon Plant regeneration Adventitious bud |
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