果品蔬菜中维生素C测定方法的研究

以2,6一二氯靛酚滴定法研究了八种酸性浸提剂对纯抗坏血酸和各种果蔬样品中维生素 C 测定值的影响。其中2%偏磷酸、偏磷酸-醋酸,2%草酸对抗坏血酸的稳定性最佳,其回收率均在96%以上,达到一定的准确度要求。三者对多种果蔬样品的维生素 C 测定平均值,经 t 测验其差异均不显著,可以互相代替使用。试样匀浆方式(捣碎法、研磨、榨汁)对维生素测定影响不大,捣碎法快速,适用于大批样品分析;对多汁的试样用榨汁法简使,计算时应以 mg/100ml 表示。捣碎时的样液比对维生素 C 测定值影响不大,可根据样品含水量多少而定,匀浆后可用不同的稀释倍数浸提,只要滴定时的试液控制抗坏血酸含量在0.2—1mg 范围内即可。白陶土对大多数深色试样有良好的脱色效果。但对抗坏血酸有吸附作用(2—4%),测定浅色试样时,尽量不用白陶土脱色。

A STUDY ON METHODS OF DETERMINING VITAMIN C IN FRUITS AND VEGETABLES

By means of 2.6-dichlorophenol indophenol titrimetic method the effect of eight extracting acid solutions on the value of pure ascorbic acid and vitamin C from various kinds of fruits and vegetables was studied.Among them 2% matephosphoric acid,matephosphoric-acetic acid and 2% oxalic acid gave the best result.The rates of recovery of standard ascorbic acid,which had been put in the sample,were more than 96%,and the accuracy of the test reached the desired level.The differences between the average quantitie...