不同挥发物诱导的合作杨叶片中POD,PPO及PAL活性变化

以1年生合作杨扦插苗为试验材料,利用茉莉酸甲酯(MeJA)分别与水杨酸甲酯(MeSA)、顺式己烯醛(cis-hexenal)、反式己烯醛(trarts-hexenal)和苯骈噻唑(benzothiazole)混合的4种挥发物熏蒸合作杨叶片,检测过氧化物酶(POD)、多酚氧化酶(PPO)及苯丙氨酸解氨酶(PAL)3种防御酶的活性变化.结果表明:经过4种混合挥发物熏蒸后,合作杨叶片中POD,PPO和PAL活性都有明显的升高,并且2种挥发物混合的诱导效果要高于单独一种挥发物,但不仅仅是每一种挥发物诱导效果的相加之和.从诱导酶活增加的效果来看,在这4种混合挥发物中,MeJA+cis-hexenal的诱导能力最高.

Activities of POD, PPO and PAL in Poplar(Populus simonii × P. pyramidalis 'Opera 8277') Leaves Exposed to Different Volatiles

The signal transfer between plants was mediated by compound signals composed of different volatiles. In order to explore the action mode of compound signals, one-year-old poplar( Populus simonii x P. pyramidalis ' Opera 8277 ) cuttings were exposed to four mixed volatiles, i.e. methyl jasmonate(MeJA) + methyl salicylate(MeSA) , MeJA + cis-hexenal, MeJA + trans-hexenal, MeJA + benzothiazole, and then the induced activities of three defensive enzymes including peroxidase (POD) , polyphenol oxidase(PPO) and phenylalanine ammonia lyase(PAL) were measured. The results showed that all of four mixed volatiles exposure resulted in higher activities of POD, PPO and PAL than only single volatile treatment in intact poplar leaves, indicating that mixed volatiles possessed stronger inducing capability. Moreover the synthetic inducing effect of mixed volatiles was more than the sum of individual volatiles. Among these four mixed volatiles, MeJA + cm-hexenal exhibited the highest inducing capability.