表达传染性法氏囊病病毒VP2基因的重组鸡痘病毒的构建及其免疫保护性研究 |
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引用本文: | 沈瑞忠,王笑梅,陈忠斌,李雁冰,于康震.表达传染性法氏囊病病毒VP2基因的重组鸡痘病毒的构建及其免疫保护性研究[J].中国预防兽医学报,1998(2). |
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作者姓名: | 沈瑞忠 王笑梅 陈忠斌 李雁冰 于康震 |
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作者单位: | 中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室 |
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摘 要: | 以脂质体转染技术构建了表达鸡传染性法氏囊病病毒(IBDV)VP2基因的重组鸡痘病毒FPV-VP2,该病毒在鸡胚成纤维细胞及鸡体内均能稳定产生子代病毒,经翅皮下5×105PFU/羽免疫1日龄SPF鸡,免疫后4周以100LD50/羽IBDV超强毒株G株攻毒,获得了5/6的保护,但不能有效预防临床发病及法氏囊受损萎缩。实验结果证明了VP2是IBDV的宿主保护性抗原,提示T细胞介导的免疫可能在IBDV的免疫中起着较为重要的作用。本研究为IBDV重组病毒疫苗研制进行了有益探索。
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关 键 词: | 传染性法氏囊病 重组鸡痘病毒 病毒活载体疫苗 |
A Recombinant Fowlpox Virus Expressing IBDV VP2 Gene Induces Protection Against Lethal Effect of IBDV |
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Abstract: | A recombinant fowlpox virus,FPV VP2,was constructed which expressed the host protective antigen VP2 of infectious bursal disease virus (IBDV)and conferred protection against IBDV G strain,a Chinese highly virulent strain of IBDV.The FPV VP2 contained the VP2 gene of IBDV under the control of fowlpox early/late promoter LP2EP2 AND the LacZ gene under the control of late promoter P11 of vaccinia virus inserted in a non essential region of FPV.Wing web inoculation of SPF chicken with FPV VP2 developed typical fowlpox lesions and induced low titre of antibody to IBDV.When challenged with IBDV G strain,5 out of 6 chickens vaccinated by FPV VP2 were protected,and the antibody levels increased rapidly as high as those in the group vaccinated by D78 strain,an attenuated vaccine strain of IBDV. |
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Keywords: | Infectious bursal disease virus Recombinant fowlpox Live virus vected vaccine |
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