甜叶悬钩子植物组织培养褐化现象控制研究

目的]寻求甜叶悬钩子组织培养中的最佳抗褐化剂。方法]以甜叶悬钩子的茎和叶为外植体，必MS为基本培养基，加入不同浓度的抗褐化剂（20％Na2S2O3、Vc和AC），研究不同抗褐化剂对甜叶悬钩子组织培养的影响。结果]AC和Vc都能对不同外植体起到抗褐化作用，在加入20％Na2S2O3的培养基上，外植体的褐化程度与Na2S2O3的浓度成正比，培养基中20％Na2S2O3的添加量达20ml时，外植体褐化最为迅速；在加入Vc的培养基上，随着Vc添加量的增加，培养基变成液体或半固体培养基，接入的外植体容易死亡；在加入AC的培养基上，AC的添加量为5g／L时，外植体的褐化率可控制在5％以下。抗褐化控制处理后，可以在诱导愈伤组织的培养基上培养出大量的愈伤组织。1结论IAC是较为理想的抗褐化剂，用量为5g／L。

Study on the Browning Control in Tissue Culture of Rubus suavissimus S. Lee

Objective] The purpose was to seek for optimum anti-browning agent in the tissue culture of Rubus suavissimus S.Lee.Method] With stems and leaves of R.suavissimus as explants, MS basic medium was used as the media by adding different concn.of anti-browning agent (20% Na2S2O3, Vc and AC) so as to research the effect of different anti-browning agent on tissue culture of R.suavissimus.Result] AC and Vc had anti-browning effect on different explants.On the medium with 20% Na2S2O3, the browning extent of explants was proportional to the concn.of Na2S2O3.When the addition of 20% Na2S2O3 amounted to 20 ml, the browning of the explant was the fastest.On the medium with Vc, with the increasing addition of Vc, the medium got into a liquid or semi-solid medium and the explants died easily.On the medium with AC, when the addition of AC was 5 g/L, the browning rate of the explants could be controlled below 5%.A large number of callus could be induced on the culture medium after the anti-browning control treatment.Conclusion]AC was the best anti-browning agent and dosage was 5 g/L.