| 基于小麦白粉病菌rDNA ITS序列的PCR分子检测 |
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| 引用本文： | 曾晓葳,骆勇,周益林,段霞瑜.基于小麦白粉病菌rDNA ITS序列的PCR分子检测[J].植物病理学报,2008,38(2):211-214. |
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| 作者姓名： | 曾晓葳 骆勇 周益林 段霞瑜 |
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| 作者单位： | 1. 中国农业科学院植物保护研究所植物病虫害生物学国家重点实验室, 北京 100094;2. 中国农业大学植物病理学系, 北京 100094;3. 美国加州大学Kearney农业研究中心, Parlier CA 93648 |
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| 基金项目： | 国家自然科学基金，国家科技支撑计划，公益性行业科研专项 |
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| 摘 要： | Wheat powdery mildew(Blumeria graminis f.sp.tritici) is the one of main wheat diseases in China.Based on the internal transcribed spacer(ITS) sequences of ribosome of B.graminis f.sp.tritici,three molecular primer pairs(F1/R,F2/R and F3/R) were designed to detect the fungal pathogen of wheat powdery mildew.The species specificity of these primers was confirmed.F1/R was demonstrated a higher sensitivity than the other two primer pairs,and could detect as low as 1 pg DNA of B.graminis f.sp.tritici.Furthermore,F1/R primer pair was used to detect the pathogen DNA extracted from wheat leaves showing chlorosis and typical symptoms of powdery mildew caused by artificial inoculation with B.graminis f.sp.tritici.The preliminary results demonstrated the usefulness of this primer pair and its potential applications in efficient detection of wheat powdery mildew pathogen from leaves with latent infections at early growth stages of wheat.
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| 关 键 词： | 小麦白粉病菌 PCR分子检测 ITS序列 rDNA 小麦病害 小麦产量 预测预报 白粉菌 |
PCR detection of Blurnoria graminis f. sp. tritici based on the sequences of rDNA ITS |
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| ZENG Xiao-wei,LUO Yong,ZHOU Yi-lin,DUAN Xia-yu.PCR detection of Blurnoria graminis f. sp. tritici based on the sequences of rDNA ITS[J].Acta Phytopathologica Sinica,2008,38(2):211-214. |
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| Authors: | ZENG Xiao-wei LUO Yong ZHOU Yi-lin DUAN Xia-yu |
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| Institution: | 1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China;2. Department of Plant Pathology, China;3. Agricultural University, Beijing 100094, China/University of California-Davis, Kearney Agricultural Center, Parlier CA 93648, USA |
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| Abstract: | Wheat powdery mildew(Blumeria graminis f.sp.tritici) is the one of main wheat diseases in China.Based on the internal transcribed spacer(ITS) sequences of ribosome of B.graminis f.sp.tritici,three molecular primer pairs(F1/R,F2/R and F3/R) were designed to detect the fungal pathogen of wheat powdery mildew.The species specificity of these primers was confirmed.F1/R was demonstrated a higher sensitivity than the other two primer pairs,and could detect as low as 1 pg DNA of B.graminis f.sp.tritici.Furthermore,F1/R primer pair was used to detect the pathogen DNA extracted from wheat leaves showing chlorosis and typical symptoms of powdery mildew caused by artificial inoculation with B.graminis f.sp.tritici.The preliminary results demonstrated the usefulness of this primer pair and its potential applications in efficient detection of wheat powdery mildew pathogen from leaves with latent infections at early growth stages of wheat. |
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| Keywords: | Blumeria graminis f sp tritici PCR molecular detection |
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