| 用超敏感银染SDS-PAGE方法绘制黄瓜、菜豆蛋白质指纹图谱 |
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| 引用本文： | 陈宏,王振英,靳阳,陈莹,付富友,彭永康.用超敏感银染SDS-PAGE方法绘制黄瓜、菜豆蛋白质指纹图谱[J].华北农学报,2000,15(4):108-111. |
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| 作者姓名： | 陈宏 王振英 靳阳 陈莹 付富友 彭永康 |
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| 作者单位： | 天津师范大学,生物系,天津,300074 |
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| 基金项目： | 天津市教委资助项目，天津市科委科研项目，98207C，983701611，， |
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| 摘 要： | 利用超敏感银染ＳＤＳ－ＰＡＧＥ方法对黄瓜胚轴和菜豆胚轴、胚根组织的全蛋白指纹图谱作了初步分析，黄瓜胚轴至少有４０条蛋白质谱带被检测到；黄豆胚轴中有５０多条、胚根６０多条蛋白质谱带被检出，谱带清晰、稳定，个体间谱型一致，且分离过程短，减少了蛋白质的部分变性和损失，结果较全面。
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| 关 键 词： | 蛋白质指纹图谱 超敏感银染SDS－PAGE 种子纯度检测 |
| 修稿时间： | 1999-12-12 |
Drawing the Protein Fingerprints of Cucumis sativus and Phaseolus vulgaris by Supersensitive Silver Stain SDS-PAGE Method |
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| CHEN Hong,WANG Zhen-ying,JIN Yang,CHEN Ying,FU Fu-you,PENG Yong-kang.Drawing the Protein Fingerprints of Cucumis sativus and Phaseolus vulgaris by Supersensitive Silver Stain SDS-PAGE Method[J].Acta Agriculturae Boreali-Sinica,2000,15(4):108-111. |
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| Authors: | CHEN Hong WANG Zhen-ying JIN Yang CHEN Ying FU Fu-you PENG Yong-kang |
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| Abstract: | In this study,total protein fingerprints of Cucumis sativus and Phaseolus vulgaris in hypocotyl and radicle tissue are analyzed primarily.The results showed that at least 40 protein bands are detected in hypocotyl of Cucumis sativus,and over 50 bands of hypocotyl and 60 bands of radicle of Phaseolus vulgaris were also detected because of more amount of protein in seedling of phaseolus vulgaris The protein bands detected were clear and stable,and there was consistency between individuals,the degree of denaturation and loss of protein decreased,time needed was shortened and whole results can be drawn. |
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| Keywords: | Total protein fingerprints Supersensitive Silver Stain SDS-PAGE Detection of purity Cucumis sativur Phaseolus vulgaris |
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