| 利用SSR标记构建贵州山区玉米杂交种的指纹库 |
| |
| 引用本文： | 曾桂萍,戴保威,余显权,田兴文.利用SSR标记构建贵州山区玉米杂交种的指纹库[J].种子,2007,26(8):45-47. |
| |
| 作者姓名： | 曾桂萍 戴保威 余显权 田兴文 |
| |
| 作者单位： | 1. 贵州大学农学院,贵阳,550025
2. 贵州省六枝特区种子公司,贵州六枝,553000 |
| |
| 摘 要： | 对贵州山区目前在生产上正在使用的西山系列玉米杂交种12份进行了SSR分析。从60对引物中筛选到稳定性好、多态性丰富的8对引物作为核心引物。用这8对引物的指纹组合构建了贵州山区西山系列玉米杂交种的DNA指纹数据库。结果表明,利用SSR技术进行玉米杂交种真实性的鉴定是可行、有效的。
|
| 关 键 词： | 玉米 杂交种 DNA指纹 |
| 文章编号： | 1001-4705（2007）08-0045-03 |
| 修稿时间： | 2007-04-28 |
SSR Fingerprinting Database of Maize Hybrid Cultivars Used in Mountain of Guizhou |
| |
| ZENG Gui-ping,DAI Bao-wei,YU Xian-quan,TIAN Xing-wen.SSR Fingerprinting Database of Maize Hybrid Cultivars Used in Mountain of Guizhou[J].Seed,2007,26(8):45-47. |
| |
| Authors: | ZENG Gui-ping DAI Bao-wei YU Xian-quan TIAN Xing-wen |
| |
| Institution: | 1. Agronomy College, Guizhou University, Guiyang 550025, China; 2. Company of seed ,Liuzhi, Guizhou 553000, China |
| |
| Abstract: | The polymorphism of 12 elite maize hybrid cultivars used in mountain of Guizhou.Were analysized by SSR molecular marker out of 60 pairs,eight SSR primers,which gave stable and polymorphic amplification profiles,were selected.A complete DNA fingerprinting database of maize hybrid cultivars used in mountain of Guizhou was constructed with amplification profiles from 8 pairs SSR primer.The experiment showed that SSR technique was practical and effective to identify the genuineness of maize hybrid cultivars. |
| |
| Keywords: | SSR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
