澳洲坚果授粉试验及花粉管生长的荧光显微观察

澳洲坚果品种HAES900的授粉试验及花粉管生长的荧光显微观察表明:HAES900×D4、HAES900×HAES695、HAES900×HAES900平均每个花序的最终座果率分别为0.56%、0.25%、0.12%,异花授粉的最终座果率比自花授粉的高出108%～367%,说明品种HAES900的异交亲和性好于自交亲和性;HAES900自花授粉的20个花序中,最终仅有20%的花序结实,说明品种HAES900可能具有部分自交不亲和性;澳洲坚果授粉后6h柱头上花粉大量萌发;异花授粉花柱基部的花粉管数量是自花授粉的2.5倍。

Pollination of Macadamia Cultivar HAES900 and Observation of the Growth of Pollen Tubes under Fluorescent Microscope

Flowers of macadamia cultivar HAES900 were pollinated and the pollen tube growth was observed under fluorescence microscopy. The combinations HAES900×D4 and HAES900×HAES695 and HAES900×HAES900 had an average final fruitsetting rate of 0.56% and 0.25% and 0.12% respectively each raceme. The cross-pollination combinations produced a final fruitsetting rate 108%～367% higher than the self-pollination combinations. These showed cross-compatibility of HAES900 is better as compared with its self- compatibility. Of the 20 self-pollinated racemes of HAES900 20% racemes set fruit finally , indicating HAES900 was partially self-incompatible. Numerous pollen grains were germinated on the stigma 6 hours after pollination. The cross-pollination combinations produced 2.5-fold more pollen tubes on the base of the flower style than the self-pollination combinations.