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金花茶两种体胚发育途径蛋白表达的双向电泳分析
引用本文:杨 旸 邓素芳 赖钟雄. 金花茶两种体胚发育途径蛋白表达的双向电泳分析[J]. 中国农学通报, 2011, 27(6): 68-73
作者姓名:杨 旸 邓素芳 赖钟雄
作者单位:1. 福建农林大学园艺植物生物工程研究所,福州,350002;泉州福源工贸发展有限公司,福建泉州,362800
2. 福建农林大学园艺植物生物工程研究所,福州,350002;福建省农业科学院农业生态研究所,福州,350013
3. 福建农林大学园艺植物生物工程研究所,福州,350002
摘    要:本试验在已经建立的金花茶(Camellia nitidissima Chi.)花状多子叶形胚发育途径的体胚循环再生系统基础上,用双向电泳的方法按发育时间顺序分析了金花茶正常体胚和花状多子叶形胚两种体胚发育途径各自不同阶段离体培养材料的蛋白表达差异以及相同发育阶段两种发育途径的蛋白表达差异。结果表明:随着体胚发育的进程,蛋白种类和总数先增多,在子叶胚阶段蛋白种类和数量最多,之后又逐渐减弱甚至消失,在成苗阶段种类最少;金花茶两种体胚发育途径不同阶段蛋白表达存在明显差异,PD(14.9kD)是金花茶花状多子叶形胚发育途径的特有蛋白,在可分化花状多子叶胚的愈伤组织中,以及从球形胚到成熟胚的各个阶段中都有表达。

关 键 词:竹笋  竹笋  微量元素  ICP-MS  微波消解  
收稿时间:2010-06-28
修稿时间:2010-12-06

The 2-DE Analyses of the Proteins Expression during the Two Somatic Embryo Development Pathways in Camellia nitidissima Chi.
DENG Su-Fang. The 2-DE Analyses of the Proteins Expression during the Two Somatic Embryo Development Pathways in Camellia nitidissima Chi.[J]. Chinese Agricultural Science Bulletin, 2011, 27(6): 68-73
Authors:DENG Su-Fang
Affiliation:Yang Yang1,2,Deng Sufang1,3,Lai Zhongxiong1(1Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou 350002,2 Quanzhou Fuyuan Industry & Development Co. Ltd.,Quanzhou Fujian 362800,3 Agricultural Ecology Institute,Fujian Academy of Agricultural Sciences,Fuzhou 350013)
Abstract:This experiment based on the established multiple cotyledons and flower-like shape(MCFS) repetitive embryogenesis system inCamellia nitidissima Chi.. The difference in the total proteins expression of the materials during the MCFS pathway and normal developmental pathway was analyzed by 2-DE. The results showed that:along with the growth advancement of somatic embryo,the protein variety and quantity increased first,and reached to the most in the cotyledon embryo stage,afterward gradually weakened,even vanis...
Keywords:Camellia nitidissima Chi.  somatic embryogenesis  embryo with multiple cotyledons and flower-like shape  2-DE  
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