| 黑木耳漆酶酶学性质分析 |
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| 引用本文： | 张利明,高玉千,贾海军,蒋瑾,张庭静,徐淑霞,张世敏,吴坤. 黑木耳漆酶酶学性质分析[J]. 山东农业科学, 2010, 0(6): 66-68 |
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| 作者姓名： | 张利明 高玉千 贾海军 蒋瑾 张庭静 徐淑霞 张世敏 吴坤 |
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| 作者单位： | 河南农业大学生命科学学院,河南,郑州,450002 |
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| 基金项目： | 河南省科技厅重点攻关项目 |
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| 摘 要： | 从12个黑木耳菌株中筛选出一株黑木耳漆酶高产菌株——地茂1号，对其进行Native—PAGE，发现其含有3种漆酶同工酶。对其发酵液进行酶学性质分析，发现地茂1号在液体培养基中第11天酶活达到最大，为382．6U／L，最适温度为50℃，最适pH值为3．2，温度及pH稳定性较好。
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| 关 键 词： | 黑木耳 漆酶 酶学性质 |
Analysis on Laccase Properties of Auricularia auricular |
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| ZHANG Li-ming,GAO Yu-qian,JIA Hai-jun,JIANG Jin,ZHANG Ting-jing,XU Shu-xia,ZHANG Shi-min,WU Kun. Analysis on Laccase Properties of Auricularia auricular[J]. Shandong Agricultural Sciences, 2010, 0(6): 66-68 |
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| Authors: | ZHANG Li-ming GAO Yu-qian JIA Hai-jun JIANG Jin ZHANG Ting-jing XU Shu-xia ZHANG Shi-min WU Kun |
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| Affiliation: | ZHANG Li-ming,GAO Yu-qian,JIA Hai-jun,JIANG Jin,ZHANG Ting-jing,XU Shu-xia,ZHANG Shi-min,WU Kun(College of Life Science,Henan Agricultural University,Zhengzhou 450002,China) |
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| Abstract: | Dimao 1 with high production of laccase was screened from twelve strains of Auricularia auricular.The native-PAGE analysis showed that it possessed three kinds of laccase isoenzymes.The analysis on the enzymatic properties showed that the laccase activity arrived at the highest level of 382.6 U/L after Dimao 1 strain was fermented for 11 days in liquid PDA medium,and the optimum temperature and pH value were 50℃ and 3.2 respectively for laccase activity.The laccase production of Dimao 1 was more stable to different temperatures and pH values. |
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| Keywords: | Auricularia auricular Laccse Enzymatic properties |
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