| 省沽油ISSR引物筛选及居群遗传多样性初探 |
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| 引用本文： | 陈龙灿,王颖,王业中,张远海,束庆龙,王勤,张良富,曹翠萍.省沽油ISSR引物筛选及居群遗传多样性初探[J].安徽农业大学学报,2014,41(3):462. |
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| 作者姓名： | 陈龙灿 王颖 王业中 张远海 束庆龙 王勤 张良富 曹翠萍 |
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| 作者单位： | 安徽农业大学林学与园林学院,合肥230036;岳西枯井园省级自然保护区,岳西246600;金寨县林业局,金寨237321 |
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| 基金项目： | 安徽农业大学稳定和引进人才科研项目（yi2011-38）资助。 |
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| 摘 要： | 测试和筛选出14条多态性较高的省沽油ISSR有效引物,并对安徽省野生省沽油的遗传多样性进行初步调查分析。共扩增出143个多态性位点,居群水平平均有效等位基因数ne为1.271,期望杂合度He为0.165,Shannon多态性信息指数I为0.257,物种水平遗传多样性参数分别为ne=1.341,He=0.227,I=0.370;居群间遗传分化系数为Gst=0.271。初步揭示了野生省沽油的遗传多样性,同时表明ISSR标记对于研究省沽油遗传多样性的适用性和可靠性,研究结果将为进一步深入研究省沽油多居群的遗传多样性和资源保护提供帮助。
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| 关 键 词： | 省沽油 ISSR标记 引物筛选 居群遗传多样性 |
ISSR primer selection and preliminary analysis of genetic diversity in natural populations of Staphylea bumalda |
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| CHEN Longcan,WANG Ying,WANG Yezhong,ZHANG Yuanhai,SHU Qinglong,WANG Qin,ZHANG Liangfu and CAO Cuiping.ISSR primer selection and preliminary analysis of genetic diversity in natural populations of Staphylea bumalda[J].Journal of Anhui Agricultural University,2014,41(3):462. |
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| Authors: | CHEN Longcan WANG Ying WANG Yezhong ZHANG Yuanhai SHU Qinglong WANG Qin ZHANG Liangfu and CAO Cuiping |
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| Institution: | School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036,School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036,Kujingyuan Provincial Nature Reserve of Yuexi County, Yuexi 246600,Forestry Bureau of Jinzhai County, Jinzhai 237321,School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036,School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036,School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036 and School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036 |
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| Abstract: | |
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| Keywords: | Staphylea bumalda ISSR markers primer selection genetic diversity |
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