Enzootic bovine leukosis application of E.L.I.S.A. technique for the detection of antibodies |
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| Authors: | P Maris P Nougayrede G Perrin |
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| Institution: | Ministère de l''Agriculture, Direction de la Qualité, Services Vétérinaires, Laboratoire National des Médicaments Vétérinaires, “La Haute-Marche”, Javené, 35300 Fougères, France |
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| Abstract: | A simple microplate method of enzyme-linked immunosorbent assay (E.L.I.S.A.) for detecting antibodies to bovine leukosis virus (B.L.V.) is described. The antigen consisted of a solution containing the two major antigens of the B.L.V. (gp 51 and p 24) obtained by a technique of purification using CN-Br activated Sepharose 4B. This E.L.I.S.A. was compared with the agar gel immunodiffusion test (A.G.I.D.T.) in a study of 545 bovine sera. The total discrepancy rate between the two tests was 11% with a better sensitivity for E.L.I.S.A. |
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| Keywords: | Bovin virus de la leucose bovine chromatographie d'affinité anticorps E L I S A I D G comparaison Bovine bovine leukosis virus affinity chromatography antibody E L I S A A G I D T comparison |
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