Different genes can be responsible for crown rot resistance at different developmental stages of wheat and barley |
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Authors: | Xueming Yang Jun Ma Haobing Li Hongxiang Ma Jinbao Yao Chunji Liu |
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Institution: | (1) CSIRO Plant Industry, 306 Carmody Road, St Lucia, QLD, 4067, Australia;(2) Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, 210014, People’s Republic of China;(3) School of Plant Biology, The University of Western Australia, Perth, WA, 6009, Australia; |
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Abstract: | Crown rot, caused by several Fusarium species, is one of the most damaging diseases in wheat and barley. Growing resistant varieties has long been recognised as
an integral part in effectively managing the disease. One of the factors hindering the progress of breeding for crown rot
resistance is the lack of a reliable and high throughput bioassay that allows rapid and accurate assessment of large numbers
of genotypes so that highly susceptible materials can be quickly rejected and potentially resistant lines identified for more
focused further assessments. We developed a method which, by growing several inoculated seedlings wrapped in a single piece
of moist paper towel, offers significant advantages over all of the existing methods. The new soil-less assay takes only about
two weeks, requires very little space, and removes variability associated with the use of soil/potting mixes. Results from
the new assay are highly reproducible and agree well with known field performances of different varieties. However, mapping
studies conducted using the new soil-less assay did not detect all of the quantitative trait loci found with a soil-based
assay. These results show that, although different resistance genes may all contribute to the performance of a variety, caution
should be used when comparing results from different assays. |
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