| 仿刺参β-actin基因的克隆及在各组织中的表达 |
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| 引用本文： | 段晶晶,李霞,周伯文,姜泽东,周贺.仿刺参β-actin基因的克隆及在各组织中的表达[J].大连水产学院学报,2009,24(2):176-180. |
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| 作者姓名： | 段晶晶 李霞 周伯文 姜泽东 周贺 |
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| 作者单位： | 大连水产学院,辽宁省高校海洋生物资源可持续利用重点实验室,辽宁,大连,116023 |
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| 基金项目： | 国家自然科学基金，辽宁省自然科学基金 |
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| 摘 要： | 参考海葵Nematoste Uavectensis在GenBank中的β-actin基因mRNA部分序列（XM_001630533）设计引物，对仿刺参Apostichopusjaponicus进行特异的PCR扩增，将扩增产物测序分析，再根据测序结果设计仿刺参专用β-actin引物ACT1／ACT2，对不同退火温度和循环次数条件下的该基因RT-PCR产物进行了对比。本研究中首次克隆了仿刺参的β-actin基因，为今后仿刺参目的基因表达的半定量分析研究奠定了基础。
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| 关 键 词： | 仿刺参 β-actin 分子内标 |
Cloning and expression of β-actin gene in tissues of sea cucumber Apostichopus japonicus |
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| DUAN Jing-jing,LI Xia,ZHOU Bo-wen,JIANG Ze-dong,ZHOU He.Cloning and expression of β-actin gene in tissues of sea cucumber Apostichopus japonicus[J].Journal of Dalian Fisheries University,2009,24(2):176-180. |
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| Authors: | DUAN Jing-jing LI Xia ZHOU Bo-wen JIANG Ze-dong ZHOU He |
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| Institution: | DUAN Jing-jing,LI Xia,ZHOU Bo-wen,JIANG Ze-dong,ZHOU He(Key Laboratory of Marine Bio-resources Sustainable Utilization in Liaoning Provinc's University,Dalian Fisheries Univ.,Dalian 116023,China) |
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| Abstract: | The β-actin primer ACT1/ACT2 of sea cucumber Apostichopus japonicus was designed according to the β-actin mRNA of sea anemone Nematostella vectensis from GenBank（ XM_001630533 ）. RT- PCR products amplified and sequenced by this pair of primer were studied under different conditions of annealing temperature and amplified cycles. It was the first β- actin gene cloned in the sea cucumber which showed that PCR product amplified by this pair of primer can be used as endogenous control for gene semi - quantitative research. |
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| Keywords: | Apostichopus japonicus β- actin endogenous molecular marker |
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