高良姜与混淆品大高良姜的rDNA ITS区序列分析

利用改良的CTAB法提取药材的基因组DNA,运用通用引物对高良姜及混淆品大高良姜rDNAITS区进行PCR扩增,对产物进行直接测序并对测序结果进行聚类分析。结果表明,经ClustalX软件序列比对后发现二者ITS的长度范围在568～576bp,有32处变异位点,主要集中在ITS1区和ITS2区,聚类分析表明高良姜与大高良姜各聚为一支,表明高良姜与大高良姜两种植物碱基序列有较大的差异。

Preliminary studies on identification of Alpinia officinarum Hance and adulterants by the PCR product of rDNA ITS sequencing

The genomic DNA of Alpinia officinarum Hance was extracted from yong leaves by an improved CTAB method, the ITS sequence was amplified by PCR with universal primers of ITS and PCR products were direct sequenced and the sequencing results were clustered. The Results showed that after amplifited and searched with Blast metheds,the results showed that eight kinds of plants with a total length ITS sequence for 568～576 bp,had 32 variable sites,mainly concentrated in the ITS1 and ITS2 area.The results of phylogenetic tree indicated the tested materials clould be grouped into two classes.