N'-硝基缩氨基胍类化合物对蚜虫的室内毒力测定及相关酶活性的影响

Su50N'-硝基-1-异丙基-2-(4-硝基苯亚甲基)氨基胍]和Su56(N'-硝基-1-烯丙基-2-正丙基氨基胍)是利用活性亚结构拼接原理合成的具有新烟碱类和缩氨基脲类杀虫剂共同结构特征的硝基缩氨基胍类化合物。为了解Su50和Su56对蚜虫的生物活性及作用机理,采用叶片浸渍法测定了其对多种蚜虫的杀虫活性,用棉叶涂抹药液后接入棉蚜Aphis gossypii Glover测定了其对棉蚜的拒食活性,并采用生化方法测定了不同亚致死浓度(LC_(10)、LC_(25))Su56对棉蚜羧酸酯酶(CarE)、乙酰胆碱酯酶(AChE)、谷胱甘肽S-转移酶(GSTs)和多功能氧化酶(MFO)的酶活性的影响。结果表明:Su50和Su56对棉蚜A.gossypii/桃蚜Myzus persicae Sulzer,苹果黄蚜Aphis citricola van der Goot和梅大尾蚜Hyalopterus amygdali Blanchard均具有一定的杀虫活性,但毒力差异不显著,其中Su50对棉蚜、桃蚜、梅大尾蚜和苹果黄蚜的LC_(50)值分别为13.0、24.2、62.6和14.3mg/L,Su56的LC_(50)值则分别为30.3、30.2、101和3.23 mg/L;同时Su50和Su56对供试棉蚜均具有一定的拒食活性。生化试验结果表明,Su56在LC_(10)和LC_(25)浓度下对棉蚜的CarE、GSTs和MFO酶活性无显著影响,但在LC_(10)浓度下对棉蚜的ACNE酶活性却有显著抑制作用。

Toxicity of N'-nitrohydrazinecarboximidamides to several aphids and the influence on the activities of related enzymes in Aphis gossypii

Su50 (N'-nitro-1-isopropyl-2-(4-nitrobenzenemethene) aminoguanidine) and Su56 (N'-nitro-1-allyl-2-n-propyl-aminoguanidine) were compounds with the feature of neonicotinoid and semicarbazone that were synthesized by splicing the active substructure. In order to determine the insecticidal activity and mechanism against aphid, Aphis gossypii Glover, Myzus persicae, Aphis citricola and Hyalopterus amygdali were chosen as the target and leaf-dip method was used. The influence of Su56 on carboxylesterase (CarE), acetylcholinesterase (AChE), glutathione S-transferase (GSTs) and multifunction oxidase (MFO) was investigated at sub lethal concentration (LC₁₀ and LC₂₅). The results showed that Su50 and Su56 had insecticidal activity against the 4 kinds of aphids, howerve, the difference was not significant. For A. gossypii, M. Persicae, H. amygdale and A. ciricola, the LC₅₀ values of Su50 were 13.0, 24.2, 62.6 and 14.3 mg/L and the LC₅₀ values of Su56 were 30.3, 30.2, 101 and 3.23 mg/L, respectively. The two compounds also showed antifeedant activities against A. gossypii. The LC₁₀ and LC₂₅ of Su56 had no significant effects on CarE, GSTs and MFO activities of A. gossypii. However, AChE was inhibited significantly treated by LC₁₀ of Su56.