| 猪、犬旋毛虫DNA限制性片段长度多态性分析 |
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| 引用本文： | 张桂红,宋铭忻,李学奇.猪、犬旋毛虫DNA限制性片段长度多态性分析[J].中国预防兽医学报,2000,22(2):146-148. |
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| 作者姓名： | 张桂红 宋铭忻 李学奇 |
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| 作者单位： | 东北农业大学动物医学院!哈尔滨150030(张桂红，宋铭忻，路义鑫)，肇东市动物检疫站!151100(李学奇，张彦和)，黑龙江省兽医卫生防疫站!150030(孙刚) |
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| 基金项目： | 黑龙江省“九五”重点攻关课题 |
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| 摘 要： | 以旋毛虫国际标准虫种Ｔ．ｓｐｉｄａｉｓ和Ｔ．ｎａｔｉｖａ作对照，应用ＤＮＡ限制性片段长度多态性（ＲＦＬＰ）技术对黑龙江省猪、大旋毛虫进行虫种鉴定。结果显示：猪旋虫和Ｔ．ｓｐｉｒｌａｉｓ酶切图谱相同；犬旋毛虫和Ｔ．ｎａｔｉｖａ酶谱一致，结果提示，黑龙江猪旋毛虫为Ｔｓｐｉｒａｌｉｓ，犬旋毛虫为Ｔ．ｎａｔｉｖａ。
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| 关 键 词： | 旋毛虫隔离种 RFLP技术 虫种鉴定 疾病防治 |
| 修稿时间： | 1999-10-08 |
Identification of 4 Trichinella isolates by Restriction Fragment Length Polymorphisms analysis |
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| ZHANG Guihong,SONG Mingxin,LI Xueqi ,Zhang Yanhe ,SUN Gang ,LU Yixin.Identification of 4 Trichinella isolates by Restriction Fragment Length Polymorphisms analysis[J].Chinese Journal of Preventive Veterinary Medicine,2000,22(2):146-148. |
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| Authors: | ZHANG Guihong SONG Mingxin LI Xueqi Zhang Yanhe SUN Gang LU Yixin |
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| Institution: | ZHANG Guihong,SONG Mingxin,LI Xueqi *,Zhang Yanhe *,SUN Gang **,LU Yixin |
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| Abstract: | Trichinella from swine and dog in Hei Longjiang p
rovince was identified on the basis of Restriction Fragment Length Polymorphisms
(RFLP)analysis,the international standard Trichinella isolatesT.spiralis and T. nativa
were used as the control.RFLP analysis of 4 Trichinella isolates indicate d that swine
isolate and T.spiralis were identical,dog isolate and T.nativa were identical,but patterns
of the two groups were different to each other.It is con cluded that Trichinella from
swine and dog belong to Trichinella spiralis and Tr ichinella nativa respectively. |
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| Keywords: | Trichinella isolates Restriction Fragment Length Polymorphisms |
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