| 亚麻单倍体抗逆基因的转化 |
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| 引用本文： | 康庆华,许修宏,李柱钢,徐涵,关凤芝,刘文萍,张利国.亚麻单倍体抗逆基因的转化[J].中国麻业,2006(6). |
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| 作者姓名： | 康庆华 许修宏 李柱钢 徐涵 关凤芝 刘文萍 张利国 |
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| 作者单位： | 东北农业大学资源与环境学院，东北农业大学资源与环境学院，黑龙江省农科院生物中心，法国图卢兹综合科学研究所，黑龙江省农科院经作所，黑龙江省农科院生物中心，黑龙江省农科院经作所 黑龙江哈尔滨150030，黑龙江省农科院经作所，黑龙江哈尔滨150086，黑龙江哈尔滨150030，黑龙江哈尔滨150086，法国图卢兹市31300，黑龙江哈尔滨150086，黑龙江哈尔滨150086，黑龙江哈尔滨150086 |
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| 摘 要： | 为提高亚麻抗逆性,尽快获得转基因纯系亚麻,本研究采用农杆菌介导的转基因技术,将抗盐和低温胁迫基因HY15CS导入单倍体亚麻,结果获得抗性再生植株24株,从15株生长健壮的再生植株中PCR检测到3株阳性植株,阳性率20%;同时进行了不同方法的单倍体检测技术的研究,确定DAPI染色可以做为快速鉴定植物体倍性常用方法。
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| 关 键 词： | 亚麻 单倍体 HY15CS 转基因 DAPI染色 |
Study on Cold and Salt Resistance Gene Tranformation of Flax Haploid |
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| KANG Qing-hua,XU Xiu-hong LI Zhu-gang,XU Han,GUAN Feng-zhi,LIU Wen-ping,ZHANG Li-guo.Study on Cold and Salt Resistance Gene Tranformation of Flax Haploid[J].Plant Fibers and Products,2006(6). |
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| Authors: | KANG Qing-hua XU Xiu-hong LI Zhu-gang XU Han GUAN Feng-zhi LIU Wen-ping ZHANG Li-guo |
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| Institution: | KANG Qing-hua1,2,XU Xiu-hong1 LI Zhu-gang3,XU Han4,GUAN Feng-zhi1,LIU Wen-ping3,ZHANG Li-guo2 |
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| Abstract: | In order to improve resistance of cold and salt of flax,and select pure transgenic flax,cold and salt resistant gene HY15 was introduced into haploid flax by Agrobacterium tumefacies,and 24 regeneration plants were obtained.The PCR analyses revealed that there were 3 transformed plants whose genomes were integrated with foreign gene HY15 sequence in 15 strong regenerated plants,and the frequency on HY15 gene integrated into flax haploid regenerated plant was 20%.DAPI stain was selected from others as a fast method to judge the chromosome ploidy. |
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| Keywords: | flax haploid HY15 gene Tranformation DAPI stain |
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