| 桦褐孔菌胞外多糖脱蛋白工艺比较研究 |
| |
| 引用本文： | 王金玲,杜文婧,王琦.桦褐孔菌胞外多糖脱蛋白工艺比较研究[J].吉林农业大学学报,2010,32(6):633-638. |
| |
| 作者姓名： | 王金玲 杜文婧 王琦 |
| |
| 作者单位： | 吉林农业大学,教育部食药用菌工程研究中心,长春,130118 |
| |
| 基金项目： | 吉林省科技发展重点项目（2009901） |
| |
| 摘 要： | 采用正交试验设计,以蛋白质脱除率和多糖损失率为指标,比较研究了Sevag法、三氯乙酸法和酶法对桦褐孔菌胞外多糖的脱蛋白工艺。结果表明:三氯乙酸法脱蛋白效果最佳,其优化工艺为三氯乙酸浓度0.5mol/L,加入量15%,振荡时间20min,静止时间40min,蛋白质脱除率为68.5%,多糖损失率为2.45%。
|
| 关 键 词： | 桦褐孔菌 胞外多糖 脱蛋白 |
| 收稿时间： | 2010-05-06 |
| 修稿时间： | 2010-06-02 |
Comparative Study of Deproteinization Process of Exo-Polysaccharide from Inonotus obliquus |
| |
| WANG Jin-ling,DU Wen-jing,WANG Qi.Comparative Study of Deproteinization Process of Exo-Polysaccharide from Inonotus obliquus[J].Journal of Jilin Agricultural University,2010,32(6):633-638. |
| |
| Authors: | WANG Jin-ling DU Wen-jing WANG Qi |
| |
| Institution: | Engineering Research Center of Edible and Medicinal Fungi of Ministry of Education, Jilin Agricultural University, Changchun 130118, China |
| |
| Abstract: | To investigate the optimal deproteinization methods from exopolysaccharide of Inonotus obliquus, Sevag, trichloracetic acid and enzymic method were compared according to the deproteinization and loss rate of exo polysaccharide by orthogonal experiments. The results showed that the effectiveness of trichloracetic acid method was the best for deproteinization of I. obliquus exo polysaccharide.Ｔhe optimal conditions of trichloracetic acid method were as follows: concentration of trichloracetic acid 0.5 mol／L, volume of trichloracetic acid 15％; vibration time 20 min, quiescing time 40 min,respectively. The deproteinization ratio and loss rate of exo polysaccharide were 68.5％ and 2.45％, respectively． |
| |
| Keywords: | deproteinization |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《吉林农业大学学报》浏览原始摘要信息 |
| 点击此处可从《吉林农业大学学报》下载免费的PDF全文 |
|
