八角科观赏植物茎尖培养技术的研究

目的]探讨八角科观赏植物组织培养及快繁技术体系。方法]以八角科植物"孩儿莲"的顶芽为研究对象,以MS培养基为基本培养基,调查了培养基组成及抗褐化剂对顶芽茎尖存活和生长发育的影响。结果]春季萌动初期的顶芽是八角科植物"孩儿莲"组织培养的最适外植体材料;灭菌条件以0.1%HgCl2溶液处理6 min为宜,而常规酒精表面消毒会影响外植体存活;适宜的初代培养基为改良MS(改良大量元素与有机成分)+1倍量抗褐化剂+2.0 mg/L 6-BA+0.5 mg/L NAA,继代培养基为改良MS(改良无机成分与有机成分)+1倍量抗褐化剂+2.0 mg/L 6-BA+0.1 mg/L NAA。结论]该研究结果为"孩儿莲"组培快繁技术的建立奠定了试验基础。

Research on Primary Culture of Shoot Tip Explants of Illciaceae Ornamental Plants

Objective] This study aimed to establish a technology system for tissue culture and rapid propagation of Illciaceae ornamental plants.Method] Effects of medium components and anti-browning agents on the survival and growth of shoot tips were investigated by using apical buds of Illciaceae plants "Haierlian" as experiment material and MS as basic medium.Result] The results showed that apical buds at the early germination period in spring were the most suitable explants for tissue culture of Illciaceae plant "Haierlian".Sterilization with 0.1% HgCl2 for 6 min had achieved the best effect,while conventional surface-sterilization with alcohol would affect the survival of explants.The optimal medium for primary culture was MS-D(with modifications in major elements and organic components)+anti-browning agents(1 time of the amount)+2.0 mg/L of 6-BA+0.5 mg/L of NAA.The optimal subculture medium was MS-F(with modifications in inorganic and organic components)+anti-browning agents(1 time of the amount)+2.0 mg/L of 6-BA+0.1 mg/L of NAA.Conclusion] This study has laid a foundation for the establishment of tissue culture and rapid propagation technology system for "Haierlian".