毛果杨1-羟基-2-甲基-2-(E)-丁烯基-4-二磷酸合酶(HDS)基因的生物信息学分析

目的]研究HDS基因间的同源性及其进化关系。方法]以毛果杨的HDS基因为研究对象,利用生物信息学软件及网站对其进行碱基分布、氨基酸组成、亲疏水性、保守区以及二级结构和三级结构的预测与分析。,并与其他10个物种的HDS基因序列进行多重比对与进化分析。结果]毛果杨HDS基因的单链mRNA序列长1 956 bp,编码由656个氨基酸组成的蛋白质,该蛋白质相对分子质量为72 937.03,其中含量最高的是Leu,为10.50%;整个多肽中疏水性氨基酸只占39.63%,有10个亲水区和6个疏水区;与其他10个物种同源性多重比对发现同源性最高达99%。结论]毛果杨HDS基因处于稳定状态,编码的蛋白为亲水性蛋白,在进化过程中是保守的;试验中获得的保守区段序列信息为新基因的克隆奠定了基础。

Bioinformatics Analysis of 4-hydroxy-3-methyl-2- buten1yl diphosphate synthase (HDS) in Populus trichocarpa

Objective] The aim was to study the homology and evolutionary relationship among HAD genes.Method] Based on HDS gene of Populus trichocarpa,bioinformatics software and website were used to predict and analyze base distribution,amino acids composition,hydrophilcity or hydrophobicity,hyperconservative region,secondary structure,and three-level structure.The results were used to compare with HDS gene sequences of other ten species and related evolution analysis was followed.Result] mRNA,single chain of HDS gene of Populus trichocarpa,was 1 956 bp in length,code was made up of proteins containing amino acids of 656,and relative molecular mass of protein was 72 937.03 with Leu of 10.50% in volume;in polypeptide,hydrophobic amino acid was only of 39.63% with hydrophilc region of 10 and hydrophobic region of 6;after many comparisons with other ten species,homology was found as high as 99%.Conclusion] HDS gene of Populus trichocarpa was quite stable and coded albumen was hydrophilic protein which was quite conservative in evolution;sequence information in conservative region gained in the test paved the way for clone of novel gene.