| 纤维弧菌的低温纤维素酶研究 |
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| 引用本文： | 刘秀华,刘建凤,卢雪梅. 纤维弧菌的低温纤维素酶研究[J]. 安徽农业科学, 2012, 40(3): 1281-1282 |
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| 作者姓名： | 刘秀华 刘建凤 卢雪梅 |
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| 作者单位： | 1. 河北大学生命科学学院,河北保定,071002
2. 山东大学微生物技术国家重点实验室,山东济南,250100 |
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| 基金项目： | 国家自然科学基金，国家重点基础研究发展计划(973计划) |
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| 摘 要： | [目的]为低温纤维素酶的开发利用提供参考。[方法]以纤维弧菌2-3-a为试验材料,对其分泌的纤维素酶的酶学性质进行研究。[结果]该纤维素酶的热稳定性较差,60℃时的半衰期为15 min。酶的最适反应温度为40℃,0℃时残留酶活力为最适反应温度时的60%;最适反应pH值为5.0,属弱酸性纤维素酶。[结论]纤维弧菌2-3-a分泌的纤维素酶属于典型的低温纤维素酶。
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| 关 键 词： | 纤维弧菌 低温纤维素酶 中温微生物 |
Study on Cold-adapted Cellulase from Cellvibrio |
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| Affiliation: | LIU Xiu-hua et al(Life Science College of Hebei University,Baoding,Hebei 071002) |
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| Abstract: | [Objective] The aim was to provide the reference for development and utilization of cold-adapted cellulase.[Method] Cellvibrio 2-3-a was taken as the tested material,the enzymatic properties of cellulase from it was studied.[Result] The thermal stability of the cellulase was poor with half-life of 15 min at 60 ℃.The optional reactive temperature of the cellulase was 40 ℃,and its enzyme activity at 0 ℃ was 60% of that at the optional reactive temperature,its optional reactive pH value was 5.0,so the cellulase was weak acid cellulase.[Conclusion] The cellulase from cellvibrio 2-3-a was the typical cold-adapted cellulase. |
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| Keywords: | Cellvibrio Cold-adapted cellulase Mesophilic microorganism |
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