| 芝樱组培与快繁研究 |
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| 引用本文： | 徐美隆,陈春伶,郭晓婧,乔改霞,刘玉娟.芝樱组培与快繁研究[J].安徽农业科学,2012,40(3):1368+1371. |
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| 作者姓名： | 徐美隆 陈春伶 郭晓婧 乔改霞 刘玉娟 |
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| 作者单位： | 国家经济林木种苗快繁工程技术研究中心,宁夏银川,750000;国家经济林木种苗快繁工程技术研究中心,宁夏银川750000;种菌生物工程国家重点实验室,宁夏银川750000 |
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| 基金项目： | 北京市都市产业科技促进项目 |
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| 摘 要： | 目的]建立芝樱的组织培养和快速繁殖技术体系,为芝樱的快速繁殖及种质资源离体保存提供依据。方法]以芝樱幼嫩茎段为外植体,筛选适合芝樱组培快繁不同生长阶段的最适培养基。结果]适合芝樱启动培养的培养基为MS+6-BA 1.0 mg/L+NAA 0.1mg/L,增殖培养基为MS+6-BA 0.5 mg/L+NAA 0.01 mg/L,生根培养基为1/2 MS+IBA 0.5 mg/L+蔗糖15 mg/L+琼脂0.7%(pH 5.8～6.0)。结论]该研究建立的芝樱组培快繁体系稳定性好,增殖速度快。
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| 关 键 词： | 芝樱 组织培养 快速繁殖 |
Study on the Cultivation and Propagation of Phlox drammondii |
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| Institution: | XU Mei-long et al (National Technology Research Center of Fast Propagation Project of Tree of Economic Value,Yingchuan,Ningxia 750000) |
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| Abstract: | Objective] The aim was to establish the tissue culture and rapid propagation system of Phlox drammondii,so as to provide basis for rapid propagation and in vitro conservation system of Phlox drammondii.Method] With the tender stems as explants,the proper culture medium formula was screened in different period of growth of Phlox drammondii in propagation by tissue culture.Result] The proper initiation culture medium was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L.The proper multiplication culture medium was MS+6-BA 0.5 mg/L+NAA 0.01 mg/L.The proper rooting culture medium was 1/2MS+IBA 0.5 mg/L+Sucrose 15 mg/L+ Agar 0.7%(pH 5.8-6.0).Conclusion] The established tissue culture and rapid propagation system for Phlox drammondii had good stability and fast propagation rate. |
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| Keywords: | Phlox drammondii Tissue culture Rapid propagation |
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