SDS-聚丙烯酰胺凝胶电泳4种染色方法的比较研究

目的]比较SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)中蛋白质的染色方法,建立美洲大蠊抗肿瘤活性部位SDS-PAGE后更为合适的染色方法。方法]以牛血清白蛋白(BSA)为标准品,采用考马斯亮蓝染色法、氯化钾染色法、钙染色法和银染色法进行比较,在此基础上,将样品美洲大蠊抗肿瘤活性部位进行SDS-PAGE电泳和染色。结果]结果表明银染法能够准确、快速、简便的对美洲大蠊抗肿瘤活性部位的SDS-PAGE进行染色。结论]为美洲大蠊药用价值的研究奠定了基础。

A Comparative Study on Four Staining Methods for Antitumor Active Fraction of Periplaneta Americana

Objective] This study aimed to compare four staining methods for proteins of SDS-polyacrylamide gel electrophoresis(SDS-PAGE) and explore a suitable staining method for the antitumor active fraction of P.americana after polyacrylamide gel electrophoresis.Method] BSA was used as the standard for the comparison of Coomassie brilliant blue staining method,potassium staining method,calcium staining method and silver staining method,on the basis,antitumor active fraction samples of P.americana were used for SDS-PAGE electrophoresis and staining.Result] The results showed that silver staining method could be accurately,quickly and easily used for SDS-PAGE staining of the antitumor active fraction of P.americana.Conclusion] This study had laid the foundation for exploring the medicinal value of P.americana.