甜瓜离体再生体系优化研究

[目的]对甜瓜离体再生体系进行优化研究。[方法]以优良甜瓜品种南湘91023为试验材料,以其子叶、下胚轴为外植体,在不同组织培养阶段添加不同种类和不同浓度的生长调节剂,以筛选出简单有效的甜瓜再生培养基配方。[结果]愈伤组织的诱导及增殖以MS+1.5 mg/L 6-BA+0.2 mg/L IAA为最佳培养基;不定芽的诱导分化以MS+1.0 mg/L 6-BA+0.2 mg/L 2,4-D为最佳培养基;幼苗的诱导生根以MS+1.0 mg/L ZT+0.2 mg/L IAA为最佳培养基,在此培养基下培养,幼苗的生根率高且根粗壮。[结论]为进一步开展甜瓜的遗传转化改良品种研究提供了保障。

Research on in vitro Regeneration System of Melon

[Objective] This study aimed to optimize the in vitro regeneration system of melon.[Method] Melon variety Nanxiang91023 was selected as experimental materials,with the cotyledons and hypocotyls as explants,different types and concentrations of growth regulators were supplemented at different stages of tissue culture,to explore the simple and effective medium formula for regeneration of melon.[Result] MS+1.5 mg/L of 6-BA+0.2 mg/L of IAA was the optimal medium for induction and proliferation of callus;MS+1.0 mg/L of 6-BA+ 0.2 mg/L of 2,4-D was the optimal medium for differentiation of adventitious buds;MS+1.0 mg/L of ZT+0.2 mg/L of IAA was the optimal medium for rooting of seedlings.On the basis of above conditions,melon seedlings had high rooting rate and strong roots.[Conclusion] This study provided a guarantee for the further genetic transformation of improved melon varieties.