啤酒废酵母酶解生产橘小实蝇引诱蛋白研究

目的]优化啤酒废酵母酶解生产橘小实蝇引诱蛋白的条件。方法]采用室外小笼子生物测定法,通过正交试验设计对啤酒废酵母酶解生产橘小实蝇引诱蛋白的影响因素(酵母浓度、酶量、水解温度、水解时间、水解pH和水解产物pH)进行了优化。结果]将酵母母液稀释3倍后加入15.0 g/kg木瓜蛋白酶,在pH 6.5、温度67.5℃条件下保温水解24 h得到的水解蛋白于pH 8.5时对橘小实蝇雄性的引诱活性最强,水解27 h对橘小实蝇雌性的引诱活性最强。结论]为开发橘小实蝇蛋白诱剂提供了理论依据。

Production of Protein Bait for Bactrocera dorsalis ( Diptera: Tephritidae) by Using Beer Waste Yeast

Objective] The aim was to optimize the production conditions of protein bait for Bactrocera dorsalis(Diptera: Tephritidae) by using beer waste yeast.Method] Production conditions of protein bait for Bactrocera dorsalis by using beer waste yeast like yeast concentration,enzyme amount,hydrolysis temperature,hydrolysis time,hydrolysis pH and hydrolysis product’ pH were optimized with orthogonal design by outdoor small-cage bioassay.Result] The best attractiveness to females and males could be attained with the processes that the third thickness mother beer waste yeast liquid was treated with 15.0 g/kg papain at 67.5 ℃ and pH 6.5 for 27 and 24 hours,respectively.The bioassays also showed that the protein hydrolysate with pH 8.5 could reach its highest attractiveness.Conclusion] The research provides theoretical basis for the development of protein bait for Bactrocera dorsalis.