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| 一株O1血清型鸡大肠埃希菌iss基因的序列分析 | |
| 引用本文： | 樊琛,王宇,王会,王亚君,李一经.一株O1血清型鸡大肠埃希菌iss基因的序列分析[J].动物医学进展,2009,30(11):80-82. |
| 作者姓名： | 樊琛 王宇 王会 王亚君 李一经 |
| 作者单位： | 1. 东北农业大学动物医学院,黑龙江哈尔滨,150030;聊城大学农学院,山东聊城,252059 2. 聊城市人民医院呼吸内科,山东聊城,252000 3. 聊城大学农学院,山东聊城,252059 4. 东北农业大学动物医学院,黑龙江哈尔滨,150030;东北林业大学野生动物学院,黑龙江哈尔滨,150040 5. 东北农业大学动物医学院,黑龙江哈尔滨,150030 |
| 摘 要： | 采用1日龄雏鸡对1株O1血清型鸡源大肠埃希菌(E.coli)的致病性进行测定,并扩增iss基因.结果表明,鸡E.coli O1对1日龄雏鸡具有较强的毒力.iss基因在致病性鸡E.coli O1中的序列与已知禽大肠埃希菌iss基因序列同源性为100%,与人源大肠埃希菌iss基因核苷酸,序列同源性为90.9%,显示了此基因具有保守性. |
| 关 键 词： | 鸡大肠埃希菌 iss基因 毒力 |
Sequnce of iss Gene in Avian E.coli Strain O1 |
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| FAN Chen,WANG Yu,WANG Hui,WANG Ya-jun,LI Yi-jing.Sequnce of iss Gene in Avian E.coli Strain O1[J].Progress In Veterinary Medicine,2009,30(11):80-82. | |
| Authors: | FAN Chen WANG Yu WANG Hui WANG Ya-jun LI Yi-jing |
| Institution: | FAN Chen1,2,WANG Yu3,WANG Hui2,WANG Ya-jun1,4,LI Yi-jing1(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin,Heilongjiang,150030,China,2.Agricultural School,Liaocheng University,Liaocheng,Shandong,252059,3.Respiratory Medicine,Liaocheng People's Hospital,252000,4.Wildlife Animal College,Northeast Forestry University,150040,China) |
| Abstract: | The virulence of avian E.coli strain O1 for one day-old chicks was detected,and the isolates was examined for the presence of iss gene using PCR techniques.Results showed that avian E.coli strain O1 was strongly virulent.The sequence of iss from O1 has 100% identity with AF042279 in GenBank and 90.9% identity with the iss gene from the human E.coli isolate.It suggested that iss gene may be highly conserved. |
| Keywords: | avian E coli iss gene virulence |
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