红汁乳菇菌种分离培养及其对马尾松苗木生长效应研究

红汁乳菇菌种分离实验表明，红汁乳菇可通过组织分离获得纯菌，不同地域的红汁乳菇子实体在相同条件下进行分离时，菌丝生长的快慢不同。红汁乳菇一级菌种培养时，菌丝在PDA、MMN、酵母葡萄糖酸钙培养基上生长较快和旺盛，菌丝在MS、查氏、蛋白胨培养基上生长缓慢，且生长很弱。用液体培养的红汁乳菇接种马尾松幼苗可形成菌根化苗，苗木菌根形成率为73．3％，且菌根明显促进马尾松苗木生长。马尾松菌根化苗可以提高马尾松苗出圃率及造林成活率，促进马尾松生长，为实现红汁乳菇的半人工栽培提供基础。

Study on Isolation Culture of Lactarius hatsutake and Its Effects on Growth of Masson Pine Nursery Stock

The results showed that PDA was a more suitable medium for isolation culture of Lactarius hatsutake.Through tissue separation, the pure mycelium could be obtained.When fruit bodies of L. hatsutake from different districts isolated at the same condition, the growth velocity of mycelium was different. Mycelia grew faster on PDA , MMN ,Yeast-glucose -gallium,while slowly and weakly on the medium of MS, Cha, and peptone. Using liquid ferment Mycelia of L. hatsutake as inoculum to nursery stock of Masson Pine , mycorrhigal seedling could be formed. Formed mycorhiza could improve the growth of Masson Pine, and achieve the aim of taming culture, which will provide a new way for multi-management of forest production.