氨和乳酸对悬浮培养的BHK-21细胞生长和胞内酶活性的影响

为了研究氨和乳酸对悬浮培养的BHK-21细胞生长和细胞内酶活性的影响,用添加了不同浓度氨和乳酸的培养基悬浮培养BHK-21细胞,间隔12 h取样,比较细胞的生长状况,检测细胞代谢过程中营养物质的消耗和细胞内琥珀酸脱氢酶的活性。结果显示,当氨和乳酸的添加浓度分别为2.5和10 mmol/L时,对细胞的生长产生弱抑制作用;随着氨和乳酸添加浓度的增加,细胞生长缓慢,营养物质消耗量降低,琥珀酸脱氢酶活性降低;在氨和乳酸添加浓度分别达到5和20 mmol/L时,细胞生长代谢受到严重的抑制,琥珀酸脱氢酶活性在整个培养过程中显著低于对照组。因此,培养液中氨和乳酸积累到一定浓度会抑制BHK-21细胞生长,影响细胞内琥珀酸脱氢酶的活性。

The Effects of Ammonia and Lactic Acid on Suspension Culture of BHK-21 Cell Growth and Intracellular Enzyme Activity

In order to research the effects of ammonia and lactic acid on suspension culture BHK-21 cell growth and intracellular enzyme activity,we cultured the BHK-21 suspension cells with the media of different concentrations of ammonia or lactic acid added,and sampled every 12 hours,determined the consumption of nutrient and vitality of succinate dehydrogenase.The results showed that when the concentration added of ammonia and lactate was below 2.5 or 10 mmol/L respectively,the ammonion and lactate had weak inhibi...