| 猪细小病毒、伪狂犬病病毒和猪圆环病毒2型多重PCR检测方法的建立及应用 |
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| 引用本文： | 孙黎,郭抗抗,王静,刘伟,曹伟伟,吕其壮,张彦明.猪细小病毒、伪狂犬病病毒和猪圆环病毒2型多重PCR检测方法的建立及应用[J].动物医学进展,2011(8):25-31. |
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| 作者姓名： | 孙黎 郭抗抗 王静 刘伟 曹伟伟 吕其壮 张彦明 |
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| 作者单位： | 西北农林科技大学动医学院; |
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| 基金项目： | 陕西省重大科技攻关项目(2006kz07-G2) |
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| 摘 要： | 根据猪细小病毒(PPV)、伪狂犬病病毒(PRV)和猪圆环病毒2型(PCV-2)的基因序列,分别选取各自的保守区段设计引物,通过反应条件的优化,建立了检测PPV、PRV和PCV-2的多重PCR方法.用建立的方法对采自陕西省部分猪场的286份病料及血样进行检测,从对临床健康猪全血样品中PPV、PRV和PCV-2的检测结果看...
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| 关 键 词： | 猪细小病毒 伪狂犬病病毒 猪圆环病毒2型 多重PCR |
Establishment and Application of a Multiplex PCR for Detecting Porcine Parvovirus,Pseudorabies Virus and Porcine circovirus type2 |
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| SUN Li,GUO Kang-kang,WANG Jing,LIU Wei,CAO Wei-wei,L Qi-zhuang,ZHANG Yan-ming.Establishment and Application of a Multiplex PCR for Detecting Porcine Parvovirus,Pseudorabies Virus and Porcine circovirus type2[J].Progress In Veterinary Medicine,2011(8):25-31. |
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| Authors: | SUN Li GUO Kang-kang WANG Jing LIU Wei CAO Wei-wei L Qi-zhuang ZHANG Yan-ming |
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| Institution: | SUN Li,GUO Kang-kang,WANG Jing,LIU Wei,CAO Wei-wei,L Qi-zhuang,ZHANG Yan-ming(College of Veterinary Medicine,Northwest A &F University,Yangling,Shaanxi,712100,China) |
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| Abstract: | A multiplex polymerase chain reaction(PCR)-based assay was developed and evaluated for its ability to simultaneously detect multiple viral infections of swine.Specific primers for detecting porcine parvovirus(PPV),pseudorabies virus(PRV) and porcine circovirus type 2(PCV-2) were designed according to the full sequences of each virus strain obtained from GenBank.A multiplex PCR was established to detect PPV,PRV and PCV-2 by optimizing the PCR programs.286 blood and tissues samples collected in clinical healt... |
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| Keywords: | Porcine parvovirus Porcine pseudorabies virus Porcine circovirus type2 multiplex PCR |
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