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| 柱花草总RNA提取方法比较 | |
| 引用本文： | 张倩茜,张伟丽,刘凤民,许修宏,庞丹丹.柱花草总RNA提取方法比较[J].草业科学,2011,28(7):1326-1330. |
| 作者姓名： | 张倩茜 张伟丽 刘凤民 许修宏 庞丹丹 |
| 作者单位： | 1. 东北农业大学资源与环境学院,黑龙江哈尔滨150030 仲恺农业工程学院生命科学学院,广东广州510225 2. 仲恺农业工程学院生命科学学院,广东广州,510225 3. 仲恺农业工程学院教学科研基地,广东广州,510225 4. 东北农业大学资源与环境学院,黑龙江哈尔滨,150030 |
| 基金项目： | 广东省科技计划项目(2010B020305011) |
| 摘 要： | 以热研2号柱花草（Stylosanthes guianensis Reyan No.2）叶片为材料，利用紫外分光光度计和凝胶电泳法比较SDS 酚抽提法、CTAB法、Trizol试剂盒和柱式植物RNAout试剂盒法提取柱花草总RNA的质量和纯度。结果表明，改良CTAB法和柱式植物RNAout试剂盒法提取的RNA的OD260 nm/OD280 nm分别是1.85和1.93，OD260 nm/OD230 nm均大于2.0。凝胶电泳结果表明，改良CTAB法及柱式植物RNAout试剂盒法均有28S rRNA和18S rRNA两条清晰的条带，且无降解。其他两种方法获得的RNA品质较差，有降解和弥散现象。将改良CTAB法和柱式植物RNAout试剂盒法提取的RNA逆转录成cDNA，cDNA能扩增出一条清晰的β actin基因片段，进一步证明了改良CTAB法和柱式植物RNAout试剂盒法提取的总RNA具有很高的纯度，其中柱式植物RNAout试剂盒法的效果好于改良CTAB法。 |
| 关 键 词： | 柱花草 总RNA 提取方法 RT PCR |
Extraction methods of total RNA from Stylosanthes guianensis |
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| ZHANG Qian-qian,ZHANG Wei-li,LIU Feng-min,XU Xiu-hong,PANG Dan-dan.Extraction methods of total RNA from Stylosanthes guianensis[J].Pratacultural Science,2011,28(7):1326-1330. | |
| Authors: | ZHANG Qian-qian ZHANG Wei-li LIU Feng-min XU Xiu-hong PANG Dan-dan |
| Institution: | ZHANG Qian-qian1,2,ZHANG Wei-li2,LIU Feng-min3,XU Xiu-hong1,PANG Dan-dan2(1.School of Resourse and Enviornment,Northest Agricultural University,Heilongjiang Harbin 150030,China,2.College of Life Sciences,Zhongkai University of Agriculture and Engineering,Guangdong Guangzhou 510225,3.Teaching and Science Reserch Base,China) |
| Abstract: | The modified SDS phenol extraction method,Modified CTAB method,common Trizol and Column Plant RNAout were used to extract total RNA in leaf of Stylosanthes guianensis Reyan No.2,respectively.The quality and quantity of total RNA from above-mentioned methods were compared to select the better methods by UV spectrometer and gel electrophoresis.This study indicated that the value of OD260 nm/OD230 nm of RNA extracted by modified CTAB method and Column Plant RNAout method were higher than 2.0 and the value of O... |
| Keywords: | Stylosanthes guianensis total RNA extraction methods RT-PCR |
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