| 犬细小病毒和犬冠状病毒双重PCR方法的建立及应用 |
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| 引用本文： | 林颖,耿庆华,付海滨,李俊环,闫明媚,李成镛,林书铭.犬细小病毒和犬冠状病毒双重PCR方法的建立及应用[J].动物医学进展,2011(10):71-74. |
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| 作者姓名： | 林颖 耿庆华 付海滨 李俊环 闫明媚 李成镛 林书铭 |
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| 作者单位： | 沈阳出入境检验检疫局;辽宁省动物疫病预防控制中心; |
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| 摘 要： | 根据GenBank中犬细小病毒(CPV)和犬冠状病毒(CCV)基因序列各设计了一对引物,经试验条件的优化,建立了检测CPV的PCR方法和CCV的RT-PCR方法,扩增目的片段大小分别为337 bp和852 bp.在此基础上建立了检测CPV和CCV双重PCR方法.该双重PCR方法能特异的扩增CPV和CCV,且分别扩增出1...
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| 关 键 词： | 犬细小病毒 犬冠状病毒 双重PCR |
Establishment and Application of Duplex PCR for CPV and CCV Detection |
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| LIN Ying,GENG Qing-hua,FU Hai-hua,LI Jun-huan,YAN Ming-mei,LI Cheng-yong,LIN Shu-ming.Establishment and Application of Duplex PCR for CPV and CCV Detection[J].Progress In Veterinary Medicine,2011(10):71-74. |
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| Authors: | LIN Ying GENG Qing-hua FU Hai-hua LI Jun-huan YAN Ming-mei LI Cheng-yong LIN Shu-ming |
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| Institution: | LIN Ying1,GENG Qing-hua1,FU Hai-hua1,LI Jun-huan1,YAN Ming-mei2,LI Cheng-yong1,LIN Shu-ming1(1.Shenyang Exit-import Inspection and Qurantin Bureau,Shenyang,Liaonig,110016,China,2.Liaoning Animal Diseases Prevention and Control Center,China) |
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| Abstract: | One pair of PCR primer were designed according to sequence of Canine parvovirus(CPV) and Canine coronavirus(CCV) from GenBank respectively,which could amplify 337 bp fragment for CPV and 852 bp fragment for CCV.The products of PCR were proved to be specific.The sensitivity of PCR of CPV and CCV showed that the method could amplify 1.08 ng and 3.2 ng nucleic acid.The result of rudimentary application showed that the method was specific,sensitive,efficient,rapid,and was a new detecting method for the mixed in... |
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| Keywords: | Canine parvovirus Canine coronavirus duplex PCR |
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