| 猪PHGPx基因真核表达重组质粒的构建与鉴定 |
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| 引用本文： | 刘平,李庆阳,谢永福,况绍祥,王林川. 猪PHGPx基因真核表达重组质粒的构建与鉴定[J]. 广东畜牧兽医科技, 2011, 36(6): 36-37,40 |
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| 作者姓名： | 刘平 李庆阳 谢永福 况绍祥 王林川 |
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| 作者单位： | 华南农业大学兽医学院,广东广州,510642 |
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| 摘 要： | 从猪睾丸中提取RNA后，采用逆转录聚合酶链式反应（RT—PCR）方法获得PHGPxcDNA。扩增的目的基因片段经琼脂糖凝胶电泳回收，与pMD18-T载体连接，转入E．coli DH5α中筛选鉴定重组子。将质粒经酶切鉴定后，把酶切下的目的基因cDNA进一步克隆到真核表达载体pGAPZ旺A中，经PCR及序列鉴定，证实插入载...
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| 关 键 词： | 猪PHGPx基因 真核载体 重组质粒 |
Construction and identification of eukaryotic expression recombinant plasmid of pig PHGPx gene |
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| Liu Ping,Li Qingyang,Xie Yongfu,Kuang Shaoxiang,Wang Linchuan. Construction and identification of eukaryotic expression recombinant plasmid of pig PHGPx gene[J]. Guangdong Journal of Animal and Veterinary Science, 2011, 36(6): 36-37,40 |
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| Authors: | Liu Ping Li Qingyang Xie Yongfu Kuang Shaoxiang Wang Linchuan |
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| Affiliation: | Liu Ping,Li Qingyang,Xie Yongfu,Kuang Shaoxiang,Wang Linchuan(College of Veterinary Medicine,South China Agricultural University,Guangzhou 510642,China) |
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| Abstract: | PHGPx cDNA was obtained from pig testicular using RT-PCR method after extracting RNA. The amplified PHGPx gene fi＇agment was connected with pMD18-T vector and transformed to E. coli DH5α . After the modification and identification of the plasmid, the PHGPx gene eDNA was cloned into eukaryotic expression vector pGAPZα A. Through PCR and sequence analysis, the fi＇agment in the vector pGAPZα A was proved to be PHGPx gene. Eukaryotic expression recombinant plasmid pGAPZα A-PHGPx cDNA was successfully constructed. It laid the foundation for the further study of pig PHGPx. |
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| Keywords: | Pig PHGPx gene eukaryotic vector recombinant plasmid |
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