赖氨酸铜对小鼠免疫功能和抗氧化酶活性的影响

试验选用19~20日龄昆明普通级雄性小鼠108只,低铜饲养20 d后,随机分成3组,每组36只。第1组小鼠饲粮中加入4.5 mg/kg硫酸铜(以铜计);第2、第3组饲粮中分别加入4.5 mg/kg和45 mg/kg赖氨酸铜(以铜计)。测定小鼠部分免疫学指标及抗氧化酶活性,以研究赖氨酸铜对小鼠免疫功能和抗氧化酶活性的影响。结果表明,赖氨酸铜对小鼠血清铜锌超氧化物歧化酶(Cu,Zn-SOD)活性和血清铜蓝蛋白(CP)活性没有显著影响。赖氨酸铜能缓解小鼠胸腺退化的趋势,促进脾脏发育,提高小鼠ANAE T淋巴细胞百分比和试验期内T淋巴细胞转化率,并能提高小鼠血清IgG含量和提高单核细胞吞噬功能的趋势。45 mg/kg和4.5 mg/kg赖氨酸铜添加组相比没有显著差异。

The Effect of Cu-Lys on the Immunity and the Anti-Oxidation Enzyme Activity in Serum of Mice

108 maleKM mice of 19~20 days were on the base of copper exhaustion for 20 days,anddivided into 3 groups with 36 per group.Feeds were supplemented with CuSO_(4) 4.5 mg/kg(counted with copper) of the first group,Cu-Lys 4.5 mg/kg(counted with copper)of the second group,Cu-Lys 45 mg/kg(counted with copper)of the third group.Some immunity index and the activity of anti-oxidation enzymes were measured to study the effect of Cu-Lys on the immunity and the activity of anti-oxidation enzyme in serum of mice.The result showed that there was no significant effect of Cu-Lys on the activity of Cu,Zn-SOD and CP.Supplemented with Cu-Lys,the trend of thymus' degeneration was decreased and growth of spleen were promoted,ANAE~( )T ratio and T lymphopoiesis were increased significantly while mice's serum IgG content and monocyte phagocytosis were increased.Compared with 4.5 mg/kg Cu-Lys treatment,45 mg/kg Cu-Lys supplement level treatment showed no significant difference in immune and anti-oxidation index.