| 不同提取方法对秦艽中龙胆苦苷提取率的影响 |
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| 引用本文： | 张兴旺,于瑞涛,梅丽娟,邵谮,陶燕铎. 不同提取方法对秦艽中龙胆苦苷提取率的影响[J]. 安徽农业科学, 2009, 37(17): 7984-7985 |
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| 作者姓名： | 张兴旺 于瑞涛 梅丽娟 邵谮 陶燕铎 |
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| 作者单位： | 1. 中国科学院西北高原生物研究所,青海西宁,810008;中国科学院研究生院,北京,100049
2. 中国科学院西北高原生物研究所,青海西宁,810008 |
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| 摘 要： | [目的]建立RP-HPLC法研究不同提取方法对秦艽中龙胆苦苷提取率的影响，为秦艽药材的合理开发利用提供理论依据。[方法]采用C18色谱柱（4．6mm×250mm，5μm），以甲醇：0．3％H3PO4=30：70（V/V）为流动相，流速：1．0ml／min，柱温：30℃，检测波长：270nm。[结果]龙胆苦苷在0．9—2．1μg范围内呈良好的线性关系，r=0．9991，平均回收率为97．6％，RSD值为1．02％。[结论]动态热回流提取法的提取效率最高。
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| 关 键 词： | 秦艽 提取方法 龙胆苦苷 提取率 |
Study on the Effects of Different Extraction Methods on the Extraction Rate of Gentiopicroside from Gentiana straminea Maxim. |
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| Affiliation: | ZHANG Xing-wang et al (Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, Qinghai 810008) |
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| Abstract: | [Objective] The research aimed to study the effects of different extraction methods on the extraction rate of gentiopicroside by establishing RP-HPLC method and provide theoretical foundation for utilization of Gentiana straminea Maxim.[Method] Experiments were performed on C18(4.6 mm×250 mm,5 μm) column,with CH3OH∶0.3% H3PO4(30∶70) as mobile phase at a flow rate of 1.0 ml/min.The column temperature was 30 ℃ and the determination wavelength was 270 nm.[Result]The linear range was 0.9-2.1 μg(r=0.999 1) and the average recovery was 97.6%(RSD=1.02%).[Conclusion] The extraction of gentiopicroside from Gentiana straminea Maxim by reflux with Magnetic Stir was the most efficient. |
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| Keywords: | Gentiana straminea Maxim. Extraction methods Gentiopicroside Extraction rate |
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