| 柱花草炭疽病菌的准确快速鉴定 |
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| 引用本文： | 杨勤,罗丽娟.柱花草炭疽病菌的准确快速鉴定[J].热带生物学报,2013,4(3):220-224. |
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| 作者姓名： | 杨勤 罗丽娟 |
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| 作者单位： | [1]海南热带生物资源可持续利用国家重点实验室,海南海口570228 [2]海南大学农学院,海南海口570228 |
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| 基金项目： | “国家牧草产业技术体系”之“热带牧草育种”（CARS-35） |
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| 摘 要： | 采用常规组织分离法分离得到2个柱花草炭疽病菌致病性菌株，利用ITS通用引物ITS1／ITS4对炭疽菌rDNA—ITS进行扩增，电泳检测得到约500bp大小的目的条带，经过生物信息学分析2个菌株均为刺盘孢属胶胞炭疽病菌，且两者之间同源性达到99％，对其侵染能力进行检测，5个柱花草品系的抗病性有所不同。
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| 关 键 词： | 柱花草 炭疽病菌 核糖体rDNA-ITS 抗性分级 |
Accurate and Rapid Identification of Colletotrichum from Stylosanthes spp. |
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| YANG Qin,LUO Lijuan.Accurate and Rapid Identification of Colletotrichum from Stylosanthes spp.[J].Journal of Tropical Biology,2013,4(3):220-224. |
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| Authors: | YANG Qin LUO Lijuan |
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| Institution: | 1. National Key Laboratory for Sustainable Utilization of Tropical Biological Resources in Hainan, Haikou 571101, China; 2. College of Agronomy, Hainan University, Haikou 571101, China) |
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| Abstract: | Anthracnose is the main plant disease of Stylosanthes. Identification of pathogen of the disease is par- tieularly important. Two pathogenic strains of anthracnose were separated by using conventional tissue isolation method, and the rDNA-ITS of the pathogens were amplified by using universal primers ITS1/ITS4. The electro- phoresis results showed that the target band was 500 bp approximately. Bioinformatics analysis showed that the two strains were clustered into Colletotrichum gloeosporioides and had 99% homology. Five Stylo species were in- oculated with the two pathogenic strains, and the result showed that these 5 stylo species were different in toler- ance to anthracnose. |
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| Keywords: | Stylosanthes spp anthracnose rDNA-ITS resistance assessment |
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